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First Detection of Virulence Factors in Escherichia coli Isolated from Urogenital Tract and Correlation with Antimicrobial Resistance at the National Public Health Laboratory, Brazzaville

Nicole Prisca Makaya Dangui Nieko, Christian Aimé Kayath, Moїse Doria Kaya-Ongoto, Duchel Jeanedvi Kinouani Kinavouidi

International Journal of Pathogen Research · pp. 8–19 · Published 14 Jan 2025

10.9734/ijpr/2025/v14i1336

Abstract

Escherichia coli is the most frequently isolated pathogen of urogenital infections. Its virulence gives it the ability to evade host defences and develop resistance to antibiotics. The aim of this work is to detect virulence factors and correlate them with antibiotic resistance. The virulence genes were detected by multiplex PCR. Susceptibility to antibiotics was tested by diffusion of agar discs. Of the 102 isolated strains, 43.14% had at least one of the three virulence factors tested. The sfa/foc gene was the most predominant, with a rate of 59.10%. The antibiotic susceptibility test showed the overall resistance of the strains tested, ranging from 20 to 80%. The correlation between antibiotic resistance and the expression of virulence factors showed that strains carrying the afa and sfa/foc genes were resistant to amoxicillin/clavulanic acid at rates of 83.3% and 61.5%, respectively; strains carrying the pap gene were 95% resistant to imipenem. Statistically significant P values were obtained, respectively, 0.001 for sfa/foc, 0.034 for afa, and 0.000 for pap. Other statistically significant results were also obtained. These factor detection rates and their antibiotic resistance profiles should lead us to question the hygiene measures to be taken to avoid contamination of the urogenital tract by these factors.

E. coli antimicrobial resistance virulence factors

References (12)

  1. 1 Aminoglycoside modifying enzymes [DOI]
  2. 2 Urinary tract infections: microbial pathogenesis, host–pathogen interactions and new treatment strategies [DOI]
  3. 3 Virulence Factors of Enteric Pathogenic Escherichia coli: A Review [DOI]
  4. 4 Urinary tract infections in adults [DOI]
  5. 5 Roles of the vagina and the vaginal microbiota in urinary tract infection: evidence from clinical correlations and experimental models [DOI]
  6. 6 Escherichia coli: An Overview of Main Characteristics [DOI]
  7. 7 Uropathogenic Escherichia coli in the high vaginal swab samples of fertile and infertile women: virulence factors, O-serogroups, and phenotyping and genotyping characterization of antibiotic resistance [DOI]
  8. 8 Detection of pap, sfa, afa, foc, and fim Adhesin-Encoding Operons in Uropathogenic Escherichia coli Isolates Collected From Patients With Urinary Tract Infection [DOI]
  9. 9 Relación entre factores de virulencia, resistencia a antibióticos y los grupos filogenéticos de Escherichia coli uropatógena en dos localidades de México [DOI]
  10. 10 Resistance to Fluoroquinolones in Pseudomonas aeruginosa from Human, Animal, Food and Environmental Origin: The Role of CrpP and Mobilizable ICEs [DOI]
  11. 11 Comparative analysis of virulence factors & biotypes of Gardnerella vaginalis isolated from the genital tract of women with & without bacterial vaginosis [DOI]
  12. 12 Phenotypic and Genotypic Characterization of Metallo-Beta-Lactamase and Extended-Spectrum Beta-Lactamase among Enterobacteria Isolated at National Public Health Laboratory of Brazzaville [DOI]

Cited by 2

Carbapenem Resistance and Metallo-β-Lactamase Production in Enterobacteriaceae Isolated from Urogenital Tract Infections in Brazzaville

N. Nieko, T. Ngoulou, D. Kaya-Ongoto · Advances in Bioscience and Biotechnology · 2025

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