First Detection of Carbapenemases-Producing Acinetobacter baumannii and Acinetobacter nosocomialis in Côte d’Ivoire
Kossia Manzan Karine Gba, Rémy Bonnin, Innocent Allepo Abe, Eric Joël TAHOU, Nicole Prisca Niego Dangui Makaya, Laurent Dortet, Nathalie Guessennd, Simon-Pierre Assanvo N’guetta, Thierry Naas
Journal of Advances in Biology & Biotechnology · pp. 10–23 · Published 2 Sep 2022
10.9734/jabb/2022/v25i630286Abstract
Carbapenem resistant Acinetobacter baumannii are spreading worldwide and represent a major health threat for immunocompromised patients. In this study, we describe for the first time NDM-producing A. baumannii and Acinetobacter nosocomialis isolates from Côte d’Ivoire, Africa. Four carbapenem-resistant Acinetobacter spp. isolates were isolated from urinary tract infections from three university hospital centers. The antimicrobial susceptibility testing and carbapenemase detection (CarbAcinetoNP) test were performed. The carbapenemases genes were screened by PCR. Resistome, MLST and phylogeny were performed using whole genome sequencing. All isolates were resistant to β-lactams, aminoglycosides and fluroquinolones. The blaNDM-1 and the blaOXA-58 genes were identified in the three A. baumannii isolates whereas A. nosocomialis carried only the blaNDM-1 gene. An amino acid change S83L was detected in GyrA in all A. baumannii isolates explaining fluoroquinolone resistance. MLST analysis revealed that all A. baumannii isolates belonged to the same sequence type, ST-2. The blaNDM-1 and blaOXA-58 genes could not be transferred, suggesting a chromosomal location. This study highlights the emergence of NDM-1- and OXA-58-producing A. baumannii isolates belonging to the pandemic clone ST-2 in Côte d’Ivoire, Africa. Comparison of these three genomes revealed that they are highly related.
References (56)
- 1 Identification of acquired antimicrobial resistance genes [DOI]
- 2 The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST) [DOI]
- 3 RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes [DOI]
- 4 Characterization of a New Metallo-β-Lactamase Gene, bla NDM-1 , and a Novel Erythromycin Esterase Gene Carried on a Unique Genetic Structure in Klebsiella pneumoniae Sequence Type 14 from India [DOI]
- 5 Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria [DOI]
- 6 Global Spread of Carbapenemase-producing Enterobacteriaceae [DOI]
- 7 Metallo-β-Lactamases: the Quiet before the Storm? [DOI]
- 8 OXA β-Lactamases [DOI]
- 9 Comparative Genomics of Multidrug Resistance in Acinetobacter baumannii [DOI]
- 10 Identification of Acinetobacter baumannii by Detection of the bla OXA-51-like Carbapenemase Gene Intrinsic to This Species [DOI]
- 11 Worldwide Dissemination of the NDM-Type Carbapenemases in Gram-Negative Bacteria [DOI]
- 12 Contribution of Acquired Carbapenem-Hydrolyzing Oxacillinases to Carbapenem Resistance in Acinetobacter baumannii [DOI]
- 13 Whole-Genome Pyrosequencing of an Epidemic Multidrug-Resistant Acinetobacter baumannii Strain Belonging to the European Clone II Group [DOI]
- 14 RND-Type Efflux Pumps in Multidrug-Resistant Clinical Isolates of Acinetobacter baumannii: Major Role for AdeABC Overexpression and AdeRS Mutations [DOI]
- 15 Coexistence of blaOXA-23 with blaNDM-1 and armA in clinical isolates of Acinetobacter baumannii from India [DOI]
- 16 New Delhi metallo-beta-lactamase (NDM-1): towards a new pandemia? [DOI]
- 17 Tn 125 -Related Acquisition of bla NDM -Like Genes in Acinetobacter baumannii [DOI]
- 18 Molecular characterization of blaNDM-1 in an Acinetobacter baumannii strain isolated in Germany in 2007 [DOI]
- 19 Genetic Structures at the Origin of Acquisition and Expression of the Carbapenem-Hydrolyzing Oxacillinase Gene bla OXA-58 in Acinetobacter baumannii [DOI]
- 20 Detection of NDM-1-Producing Klebsiella pneumoniae in Kenya [DOI]
- 21 Mutation in the gyrA gene of quinolone-resistant clinical isolates of Acinetobacter baumannii [DOI]
- 22 Evolution of AbaR-type genomic resistance islands in multiply antibiotic-resistant Acinetobacter baumannii [DOI]
- 23 Expression of the Resistance-Nodulation-Cell Division Pump AdeIJK in Acinetobacter baumannii Is Regulated by AdeN, a TetR-Type Regulator [DOI]
- 24 Rapidec Carba NP Test for Rapid Detection of Carbapenemase Producers [DOI]
- 25 Quantitative Contributions of Target Alteration and Decreased Drug Accumulation to Pseudomonas aeruginosa Fluoroquinolone Resistance [DOI]
- 26 Dissemination of New Delhi metallo-β-lactamase-1-producing Acinetobacter baumannii in Europe [DOI]
- 27 Emergence of carbapenem resistance in Acinetobacter baumannii in the Czech Republic is associated with the spread of multidrug-resistant strains of European clone II [DOI]
- 28 Diversity and Evolution of AbaR Genomic Resistance Islands in Acinetobacter baumannii Strains of European Clone I [DOI]
- 29 Prevalence of carbapenemase-encoding genes including New Delhi metallo-β-lactamase in Acinetobacter species, Algeria [DOI]
- 30 NDM-5 Carbapenemase-Encoding Gene in Multidrug-Resistant Clinical Isolates of Escherichia coli from Algeria [DOI]
- 31 First report of 16S rRNA methylase ArmA-producing Acinetobacter baumannii and rapid spread of metallo-β-lactamase NDM-1 in Algerian hospitals [DOI]
- 32 Genomewide Analysis of Divergence of Antibiotic Resistance Determinants in Closely Related Isolates of Acinetobacter baumannii [DOI]
- 33 Global Comparison of the Membrane Subproteomes between a Multidrug-Resistant Acinetobacter baumannii Strain and a Reference Strain [DOI]
- 34 Epidemiology of the Acinetobacter-derived cephalosporinase, carbapenem-hydrolysing oxacillinase and metallo-β-lactamase genes, and of common insertion sequences, in epidemic clones of Acinetobacter baumannii from Spain [DOI]
- 35 First report of NDM-1-producing Acinetobacter baumannii in East Africa [DOI]
- 36 First Report of NDM-1-Producing Acinetobacter baumannii Sequence Type 25 in Brazil [DOI]
- 37 Emergence of carbapenemase-producing Gram-negative bacteria in Saint Petersburg, Russia [DOI]
- 38 Occurrence of OXA-48 and NDM-1 carbapenemase-producing Klebsiella pneumoniae in a Moroccan university hospital in Casablanca, Morocco [DOI]
- 39 Alterations of gyrA, gyrB, and parC and Activity of Efflux Pump in Fluoroquinolone-resistant Acinetobacter baumannii [DOI]
- 40 New Delhi Metallo-β-Lactamase-Producing Acinetobacter Baumannii : A Novel Paradigm for Spreading Antibiotic Resistance Genes [DOI]
- 41 Characterization of NDM-1- and OXA-23-producing Acinetobacter baumannii isolates from inanimate surfaces in a hospital environment in Algeria [DOI]
- 42 High Prevalence of bla NDM-1 Carbapenemase-Encoding Gene and 16S rRNA armA Methyltransferase Gene among Acinetobacter baumannii Clinical Isolates in Egypt [DOI]
- 43 Overexpression of the adeB Gene in Clinical Isolates of Tigecycline-Nonsusceptible Acinetobacter baumannii without Insertion Mutations in adeRS [DOI]
- 44 Emergence of NDM-producing non-baumannii Acinetobacter spp. isolated from China [DOI]
- 45 Epidemiology and clonality of carbapenem-resistant Acinetobacter baumannii from an intensive care unit in Palermo, Italy [DOI]
- 46 Dissemination of clonal complex 2 Acinetobacter baumannii strains co-producing carbapenemases and 16S rRNA methylase ArmA in Vietnam [DOI]
- 47 Chromosomal Integration of a Cephalosporinase Gene from Acinetobacter baumannii into Oligella urethralis as a Source of Acquired Resistance to β-Lactams [DOI]
- 48 Novel gyrA and parC point mutations in two strains of Acinetobacter baumannii resistant to ciprofloxacin [DOI]
- 49 AbaR-type transposon structures in Acinetobacter baumannii [DOI]
- 50 Isolation of multiple carbapenemase-producing Gram-negative bacilli from a patient recently hospitalized in Nigeria [DOI]
- 51 Beta-lactamase database (BLDB) – structure and function [DOI]
- 52 Carbapenem Resistance in Acinetobacter baumannii and Other Acinetobacter spp. Causing Neonatal Sepsis: Focus on NDM-1 and Its Linkage to ISAba125 [DOI]
- 53 Increase in the Prevalence of Resistance Determinants to Trimethoprim/Sulfamethoxazole in Clinical Stenotrophomonas maltophilia Isolates in China [DOI]
- 54 Emergence of NDM-1-producing Acinetobacter baumannii in Belgium [DOI]
- 55 Genetic characterisation of carbapenem-resistant Gram-negative bacteria isolated from the University Hospital Mohamed Boudiaf in Ouargla, southern Algeria [DOI]
- 56 Emergence of New Delhi metallo-beta-lactamase 1 and other carbapenemase-producing Acinetobacter calcoaceticus-baumannii complex among patients in hospitals in Ha Noi, Viet Nam [DOI]
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