In vitro Assessment the Antifungal Activity of Dissotis multiflora (Melastomataceae) and Paullinia pinnata (Sapindaceae) Leaves Extracts on Candida Species - Experimental Study

Abstract

Aims: The aim of this study was to evaluate in vitro the antifungal activity of Dissotis multiflora (Melastomataceae) and Paullinia pinnata (Sapindaceae) leaves extracts on six species of Candida. Study Design: This study was an experimental study. Duration and Place of the Study: Between March to August 2017, Department of Microbiology, Microbiology laboratory, University of Yaoundé I. Bacteriology laboratory, Yaoundé University Teaching Hospital (YUHC). Methodology: The fungal strains were isolated from vaginal swab women at the sampling unit of the YUHC. The identification test blastosis and gallery method allowed to differentiate Candida albicans ATCC37037 to Candida krusei, Candida tropicalis, Candida parapsilosis, Candida haemolinii and Candida lipolytica in the Bacteriology laboratory of the YUHC. C. albicans ATCC 37037 came from the Microbiology laboratory of the University of Yaoundé I. The antifungal activity of extracts was carried out on agar medium using (aromatogram) and microdilution method. The effect of the combination of methanolic fractions were assess by the chessboard method. Results: Phytochemical analysis of gude extracts of D. multiflora and P. pinnata revealed the presence of secondary metabolites such as phenols, tannins, anthraquinones, alkaloids, saponins, steroids and flavonoids in both extracts. In general, all of six fungal strains were susceptible to different extracts and fractions with inhibition diameters ranging from 10.33 mm for methanolic fraction of D. multiflorao C. parapsilosis to 19 mm for the same fraction on C. haemolinii). Both the MICs and the MFCs of actives extracts ranged respectively from 0,78 to 12,5 mg / ml and 1,56 to 25 mg/ml, the majority being fungicidal. The combinations showed significant antifungal activity compared to those of the fractions taken individually, especially with MICs reductions of the order to 75%. Conclusion: The antimicrobial activities of the molecules present in our two extracts could justify their use in traditional medicine in the treatment of candidiasis.