Skip to content
Research Article Open access CC BY 4.0

Expression of PDCD1 (PD-1) Gene among Non-small Cell Lung Cancer (NSCLC) Patients with Real-Time PCR Application

Seyed Mohammad Amin Ahmadpanah, Mohammadreza Ghanbari, Seyed Alireza Janani, Fahimeh Nemati

Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 1–9 · Published 19 Jul 2019

10.9734/ajbgmb/2019/v2i230055

Abstract

Background: PD-L1 is the main ligand is expressed on many tumors including lung cancer and is expressed in hematopoietic cells and various leukemia. The aim of this study was to evaluate the expression of PD-1 gene and the evaluation of cancerous grades of NSCLC and its subclasses from lung cancer patients in Tehran hospitals using Real-Time PCR. Materials and Methods: A total of 35 clinical samples were collected from patients with NSCLC-derived lung cancer from three hospitals in Tehran (Khatam Hospital, Athiyah Hospital, and Masih Hospital). Of the 35 samples collected in 2017, 20% of the patients were women and 80% of them were male. The range of patients’ age spectrum was 37 - 80 years. The disease grade of the patients in this study was varied and 22 different grades among them. To investigate the PDCD-1 gene expression level, after extraction of RNA and cDNA synthesis the Real-Time PCR was done and the expression of the gene was investigated. Results: The highest grade was IIIa which contained 6 patients (17.1%). 74% of adenocarcinoma cases were in T-categories of lung cancer and 25% of patients were in grade IIIa. Patients with the grade of T3 were observed in 4 samples, 2 had adenocarcinoma and 2 with SCC with age range of 55 -62 years. The results showed that the expression of PDCD-1 increased 2.46 Fold more in patients with lung cancer than NSCLC. Conclusion: The results of this study showed that there is a significant relationship between the PDCD1 or PD-1 expression of NSCLC-type lung cancer compared with healthy individuals, and using the RT-PCR for ease and rapidity it can be proved.

Lung cancer NSCLC PDCD-1 gene PD-1 gene Real-Time PCR.

References (30)

  1. 1 Engagement of the Pd-1 Immunoinhibitory Receptor by a Novel B7 Family Member Leads to Negative Regulation of Lymphocyte Activation [DOI]
  2. 2 Tumor-associated B7-H1 promotes T-cell apoptosis: A potential mechanism of immune evasion [DOI]
  3. 3 The expanding universe of T-cell subsets: Th1, Th2 and more [DOI]
  4. 4 Induced expression of PD‐1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. [DOI]
  5. 5 Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade [DOI]
  6. 6 The Consensus Coding Sequences of Human Breast and Colorectal Cancers [DOI]
  7. 7 PD-L2 is a second ligand for PD-1 and inhibits T cell activation [DOI]
  8. 8 Development of Lupus-like Autoimmune Diseases by Disruption of the PD-1 Gene Encoding an ITIM Motif-Carrying Immunoreceptor [DOI]
  9. 9 B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion [DOI]
  10. 10 CTLA-4 and PD-1 Receptors Inhibit T-Cell Activation by Distinct Mechanisms [DOI]
  11. 11 Blockade of B7-H1 and PD-1 by Monoclonal Antibodies Potentiates Cancer Therapeutic Immunity [DOI]
  12. 12 Expression of Programmed Death 1 Ligands by Murine T Cells and APC [DOI]
  13. 13 Non-Small Cell Lung Cancer [DOI]
  14. 14 PD-1 immunoreceptor inhibits B cell receptor-mediated signaling by recruiting src homology 2-domain-containing tyrosine phosphatase 2 to phosphotyrosine [DOI]
  15. 15 PD‐1 inhibits T‐cell receptor induced phosphorylation of the ZAP70/CD3ζ signalosome and downstream signaling to PKCθ [DOI]
  16. 16 Non-small-cell lung cancer [DOI]
  17. 17 Immunohistochemical localization of programmed death-1 ligand-1 (PD-L1) in gastric carcinoma and its clinical significance [DOI]
  18. 18 Structural and Functional Analysis of the Costimulatory Receptor Programmed Death-1 [DOI]
  19. 19 At the Bench: Preclinical rationale for CTLA-4 and PD-1 blockade as cancer immunotherapy [DOI]
  20. 20 PD-1 and its ligands are important immune checkpoints in cancer [DOI]
  21. 21 The dual-function CD150 receptor subfamily: the viral attraction [DOI]
  22. 22 Genetic and Epigenetic Regulation of PD-1 Expression [DOI]
  23. 23 New regulatory co-receptors: inducible co-stimulator and PD-1 [DOI]
  24. 24 Pulmonary adenocarcinomas: classification and reporting [DOI]
  25. 25 Regulated Compartmentalization of Programmed Cell Death-1 Discriminates CD4+CD25+ Resting Regulatory T Cells from Activated T Cells [DOI]
  26. 26 Treatment of advanced squamous cell carcinoma of the lung: a review. [DOI]
  27. 27 Association of PDCD1 and CTLA-4 Gene Expression with Clinicopathological Factors and Survival in Non–Small-Cell Lung Cancer: Results from a Large and Pooled Microarray Database [DOI]
  28. 28 Current Adoptive Immunotherapy in Non-Small Cell Lung Cancer and Potential Influence of Therapy Outcome [DOI]
  29. 29 Structural and Functional Analysis of the Costimulatory Receptor Programmed Death-1 [DOI]
  30. 30 Non-Small-Cell Lung Cancer [DOI]

Cited by 3

Potential Role of Phytochemical Extract from Saffron in Development of Functional Foods and Protection of Brain‐Related Disorders

Zahra Maqbool, Muhammad Sajid Arshad, Anwar Ali · Oxidative Medicine and Cellular Longevity · 2022

Showing 1 of 3 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

60

Page views

0

PDF downloads

23

Outbound clicks

3

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.