Skip to content
Research Article Open access CC BY 4.0

Exploring the Word of Thin-Layer Chromatography: A Review

Khalid Hameed, Muhammad Shoaib Khan, Ayesha Fatima, Syed Mudassir Shah, Muhammad Ali Abdullah

Asian Journal of Applied Chemistry Research · pp. 23–38 · Published 17 Oct 2023

10.9734/ajacr/2023/v14i3268

Abstract

A common analytical method in chemistry for separating and identifying mixture ingredients is called thin-layer chromatography (TLC).  A thin coating of stationary phase, often silica gel or alumina, is put into a sample, which is then placed on a flat substrate like a glass plate. The sample is then exposed to a mobile phase, often a solvent, that follows capillary action through the stationary phase. Thin-layer chromatography may be used to determine the chemicals that are present in a particular sample and track the development of a reaction. Additionally, similar substances in a mixture may be separated using TLC. Thin-layer chromatography is the favoured technique in many standard procedures in environmental chemistry, industrial chemistry, dye purity, plant material, and herbal analysis. Without having to read the whole report, it aids readers in rapidly grasping the size and importance of the study.

Chromatography TLC stationary phase mobile phase

References (43)

  1. 1 Automatic Recording Apparatus for Use in Chromatography of Amino Acids [DOI]
  2. 2 Resonance Absorption by Nuclear Magnetic Moments in a Solid [DOI]
  3. 3 Qualitative analysis of proteins: a partition chromatographic method using paper [DOI]
  4. 4 Analytical separation and detection methods for flavonoids [DOI]
  5. 5 Simultaneous Determination of All Polyphenols in Vegetables, Fruits, and Teas [DOI]
  6. 6 Differentiation of Mycobacterium, Nocardia, and Related Taxa by Thin-layer Chromatographic Analysis of Whole-organism Methanolysates [DOI]
  7. 7 Fast analysis in liquid chromatography using small particle size and high pressure [DOI]
  8. 8 Methods for Analysis of Conjugated Linoleic Acids and trans-18:1 Isomers in Dairy Fats by Using a Combination of Gas Chromatography, Silver-Ion Thin-Layer Chromatography/Gas Chromatography, and Silver-Ion Liquid Chromatography [DOI]
  9. 9 High-Performance Thin-Layer Chromatography (HPTLC) [DOI]
  10. 10 Thin-layer chromatography with biological detection in phytochemistry [DOI]
  11. 11 Qualitative determination of false‐positive effects in the acetylcholinesterase assay using thin layer chromatography [DOI]
  12. 12 Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010) [DOI]
  13. 13 High-performance thin-layer chromatography and densitometric determination of brain ganglioside compositions of several species [DOI]
  14. 14 Analysis of brain lipids by high performance thin-layer chromatography and densitometry. [DOI]
  15. 15 Profiles of photosynthetic pigments in the ocean using thin-layer chromatography [DOI]
  16. 16 Quantification in the subnanomolar range of phospholipids and neutral lipids by monodimensional thin-layer chromatography and image analysis [DOI]
  17. 17 New planar liquid chromatographic technique: overpressured thin-layer chromatography [DOI]
  18. 18 Detection of Mycotoxins by Thin-Layer Chromatography: Application to Screening of Fungal Extracts [DOI]
  19. 19 Role of advances in chromatographic techniques in phytochemistry [DOI]
  20. 20 Methods for the analysis of the saffron metabolites crocin, crocetins, picrocrocin and safranal for the determination of the quality of the spice using thin-layer chromatography, high-performance liquid chromatography and gas chromatography [DOI]
  21. 21 Development of a simplified densitometer for the determination of aflatoxins by thin-layer chromatography [DOI]
  22. 22 Thin Layer Chromatography: A Tool of Biotechnology for Isolation of Bioactive Compounds from Medicinal Plants
  23. 23 Separation of lipid classes by thin-layer chromatography [DOI]
  24. 24 The state of the art in thin-layer chromatography–mass spectrometry: a critical appraisal [DOI]
  25. 25 Carotenoid composition of Rosa canina fruits determined by thin-layer chromatography and high-performance liquid chromatography [DOI]
  26. 26 Analysis of Some Natural Antioxidants by Thin‐Layer Chromatography and High Performance Thin‐Layer Chromatography [DOI]
  27. 27 Quantitative analysis for phenolic acids by thin-layer chromatography [DOI]
  28. 28 Thin Layer Chromatography [DOI]
  29. 29 An improved method for thin-layer chromatography of nucleotide mixtures containing32P-labeled orthophosphate [DOI]
  30. 30 High-performance displacement chromatography [DOI]
  31. 31 Directly Coupled HPLC-NMR and Its Application to Drug Metabolism [DOI]
  32. 32 Capillary electrophoresis: A new technique in the analysis of plant secondary metabolites [DOI]
  33. 33 Instrumental Thin-Layer Chromatography [DOI]
  34. 34 Lipid analysis by quantitative thin‐layer chromatography [DOI]
  35. 35 Thin-Layer Chromatography. Recent Developments in Equipment and Applications [DOI]
  36. 36 Displacement chromatography on synthetic ion-exchange resins. 8. A systematic method for the separation of amino-acids [DOI]
  37. 37 Column Liquid Chromatography: Equipment and Instrumentation [DOI]
  38. 38 Displacement chromatography on synthetic ion-exchange resins. 3. Fractionation of a protein hydrolysate [DOI]
  39. 39 International Journal of Pharmaceutical Sciences Review and Research [DOI]
  40. 40 Plant drug analysis: a thin layer chromatography atlas [DOI]
  41. 41 Applied thin-layer chromatography: best practice and avoidance of mistakes [DOI]
  42. 42 Thin Layer Chromatography in Drug Analysis [DOI]
  43. 43 Modern thin-layer chromatography [DOI]

Cited by 12

TLC in the Analysis of Plant Material

Maria Zych, Alina Pyka-Pająk · Processes · 2025

Recent advances in the analytical methods for quantitative determination of antioxidants in food matrices

Nikhil Dnyaneshwar Patil, Aarti Bains, Kandi Sridhar · Food Chemistry · 2025

Phytochemical analysis techniques in food systems—methods and advances

Keke Xiao, Paraskevi Paximada · Phytoceuticals in Food for Health and Wellness · 2026

Preparatif Kromatografi: Temel Prensipleri, Uygulamaları ve Analitik Kromatografiden Ayrıntılı Değerlendirilmesi

Sirin Oba · Turkish Journal of Agriculture - Food Science and Technology · 2025

Showing 5 of 12 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

13

Page views

0

PDF downloads

1

Outbound clicks

12

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.