Exploring the Intricacies and Functionalities of Galactose Oxidase: Structural Nuances, Catalytic Behaviors, and Prospects in Bio-electrocatalysis
Nneka Damola Ajayi, Samson Abidemi Ajayi, Oluwaseun Oladeji Olaniyi
Asian Journal of Chemical Sciences · pp. 19–28 · Published 4 Jan 2024
10.9734/ajocs/2024/v14i1282Abstract
Galactose Oxidase, also known as GOase, is an enzyme found mostly in Fusarium graminearum, Dactylium dendroides, and Gibberella fujikuroi. GOase, containing copper, serves catalytic functions in oxidizing substrates and primary alcohols such as d-galactose, benzyl alcohol derivatives, and dihydroxyacetone. The catalytic property of galactose oxidase that differentiates it from other enzymes is its cofactor consisting of a Cu (II)-bound Cys-Tyr* radical. The cofactor is vital for enabling regioselective oxidation. The application of galactose oxidase (GOase) covers several fields such as enzymatic synthesis, biosensors development, and processes of diagnosis. Galactose oxidase (GOase) was discovered to have a crystallographic structure by X-ray diffraction, which revealed an active site containing copper ions displaying relatively square pyramidal geometry. There are three unique structural and functional domains of the enzyme GOase. The domains include Tyr495 and a covalent bond between Cys228 and Tyr272 serving as equatorial and axial ligands, respectively. The mechanism of catalysis covers three different oxidation states, which include the active state containing Cu (II)-radical, the intermediate state containing Cu (II)-tyrosine, and the Cu(I)-tyrosine state. The cycle of catalysis that has been posited comprises several phases which are: (i) substrate binding, (ii) the transfer of proton (iii) the transfer of hydrogen atom, and (iv) subsequent oxidation steps. These phases eventually yield the synthesis of aldehyde and hydrogen peroxide. Galactose oxidase’s (GOase) mechanism of catalysis has been studied thoroughly via extensive research focusing on explaining the ping-pong mechanism that occurs in both oxidative and reductive half-reactions. The processes of activating and reactivating galactose (GOase) involve the transfer of electrons in which horseradish peroxidase (HRP) serves as an activator. The electrochemical investigations provide evidence of the electrochemical activation and reactivation of GOase in the presence of mediators. This comprehensive review enhances the comprehension of the structural complexities, catalytic mechanisms, and bio-electrocatalytic potential of GOase, thereby establishing a basis for future investigations and developments in technology.
References (47)
- 1 The Proceedings of the National Academy of Sciences [DOI]
- 2 Biochemical and Biophysical Research Communications [DOI]
- 3 Archives of Biochemistry and Biophysics [DOI]
- 4 Oxygen radicals, nitric oxide, and peroxynitrite: Redox pathways in molecular medicine [DOI]
- 5 Investigating and Exploiting the Electrocatalytic Properties of Hydrogenases [DOI]
- 6 Direct electron transfer between copper-containing proteins and electrodes [DOI]
- 7 Ribonucleotide reductase and cancer: biological mechanisms and targeted therapies [DOI]
- 8 Mechanisms of Cytochrome P450-Catalyzed Oxidations [DOI]
- 9 Crystal Structure of a Free Radical Enzyme, Galactose Oxidase [DOI]
- 10 Protein Expression and Purification [DOI]
- 11 Activation of dioxygen by copper metalloproteins and insights from model complexes [DOI]
- 12 The radical chemistry of galactose oxidase [DOI]
- 13 Designing Artificial Metalloenzymes by Tuning of the Environment beyond the Primary Coordination Sphere [DOI]
- 14 Iron and cobalt catalysis: new perspectives in synthetic radical chemistry [DOI]
- 15 Exploring oxidative modifications of tyrosine: An update on mechanisms of formation, advances in analysis and biological consequences [DOI]
- 16 Photolyase: Dynamics and electron-transfer mechanisms of DNA repair [DOI]
- 17 Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering [DOI]
- 18 Process Requirements of Galactose Oxidase Catalyzed Oxidation of Alcohols [DOI]
- 19 The active site of galactose oxidase. [DOI]
- 20 Catalytic Mechanism of Galactose Oxidase: A Theoretical Study [DOI]
- 21 Ligand interactions with galactose oxidase: mechanistic insights [DOI]
- 22 Catalytic Reaction Profile for Alcohol Oxidation by Galactose Oxidase [DOI]
- 23 Prostaglandin H synthase: Resolved and unresolved mechanistic issues [DOI]
- 24 Enzyme-based amperometric galactose biosensors: a review [DOI]
- 25 Expression, purification, and characterization of galactose oxidase of Fusarium sambucinum in E. coli [DOI]
- 26 Electrochemical Activation of Galactose Oxidase: Mechanistic Studies and Synthetic Applications [DOI]
- 27 Novel coreactant modifier-based amplified electrochemiluminescence sensing method for point-of-care diagnostics of galactose [DOI]
- 28 Determination of biocatalytic parameters of a copper radical oxidase using real-time reaction progress monitoring [DOI]
- 29 The Mechanism of Inhibition of Pyruvate Formate Lyase by Methacrylate [DOI]
- 30 Enhanced Fructose Oxidase Activity in a Galactose Oxidase Variant [DOI]
- 31 A Galactose Oxidase Biosensor Based on Graphene Composite Film for the Determination of Galactose and Dihydroxyacetone [DOI]
- 32 Organic radical reactions confined to containers in supramolecular systems [DOI]
- 33 Structure and mechanism of galactose oxidase: catalytic role of tyrosine 495 [DOI]
- 34 On the role of superoxide radical in the mechanism of action of galactose oxidase [DOI]
- 35 Insight into the reaction mechanism of lipoyl synthase: a QM/MM study [DOI]
- 36 Active-Site Engineering Switches Carbohydrate Regiospecificity in a Fungal Copper Radical Oxidase [DOI]
- 37 Electrochemical studies of galactose oxidase [DOI]
- 38 Thiols as Mechanistic Probes for Catalysis by the Free Radical Enzyme Galactose Oxidase [DOI]
- 39 o,o-Dityrosine in native and horseradish peroxidase-activated galactose oxidase [DOI]
- 40 Properties of the Trp290His variant of Fusarium NRRL 2903 galactose oxidase: interactions of the GOasesemi state with different buffers, its redox activity and ability to bind azide [DOI]
- 41 Indirect evidence of direct electron communication between the active site of galactose oxidase and a graphite electrode [DOI]
- 42 Oxygen reactions of the copper oxidases [DOI]
- 43 Probing structural and catalytic characteristics of galactose oxidase confined in nanoscale chemical environments [DOI]
- 44 Free Radical Catalysis by Galactose Oxidase [DOI]
- 45 Oxidation rates of some desialylated glycoproteins by galactose oxidase [DOI]
- 46 Investigating and Exploiting the Electrocatalytic Properties of Hydrogenases [DOI]
- 47 JBIC Journal of Biological Inorganic Chemistry [DOI]
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