Skip to content
Research Article Open access CC BY 4.0

Exploring the In-vitro Antibacterial Efficacy of Aqueous Extracts from Leaves, Bark, and Stem of Celtis timorensis Span: A Comprehensive Study

GDS Pushpika, RS Maddumage, RMHKK Rajapaksha, YN Wickramaratne, SP Senanayake, ARN Silva

Asian Plant Research Journal · pp. 14–21 · Published 24 Feb 2024

10.9734/aprj/2024/v12i2244

Abstract

The current study evaluated the in-vitro antibacterial activity of Celtis timorensis Span. (C. timorensis) against Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), and Staphylococcus aureus (ATCC 25923) in response to the global healthcare challenge of infectious diseases caused by Multidrug Resistance organisms. The study was motivated by the inappropriate use of existing antimicrobials and the insufficient discovery of new agents contributing to this crisis. Drawing inspiration from historical evidence in plant-based Ayurveda and traditional medicine, the study focused on C. timorensis, a plant known for its historical use in treating infectious diseases. Crude extracts from the leaves, bark, and stem of C. timorensis were prepared using the cold maceration process, and their antibacterial activity was assessed against the aforementioned bacterial strains. The cylinder plate method was employed to measure zones of inhibition at various concentrations (250 µg/ml, 500 µg/ml, 750 µg/ml, and 1000 µg/ml). Positive and negative controls included Gentamicin and distilled water respectively. The diameter of inhibition zones was measured after 24 hours of incubation. Results indicated positive antibacterial effects of all three extracts against E. coli (ATCC 25922) and P. aeruginosa (ATCC 27853). Notably, aqueous extraction from the stem exhibited the highest inhibitory zones against E. coli and P. aeruginosa. However, none of the concentrations of the extracts showed positive antibacterial effects against S. aureus (ATCC 25923). Statistical analysis confirmed the significance of the observed antibacterial activity against E. coli and P. aeruginosa. Dose-response study results highlighted the efficacy and potency of aqueous extractions from bark and leaves against Escherichia coli. In contrast, leaves and bark demonstrated the highest efficacy and potency, respectively against Pseudomonas aeruginosa. In conclusion, the study scientifically validated the hypotheses formulated at the study's outset, utilizing evidence from plant-based Ayurveda and traditional medicine. The findings underscore the potential of C. timorensis as a source of antibacterial agents in the context of addressing multi-drug resistance-related infectious diseases.

Celtis timorensis Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus antibacterial activity

References (13)

  1. 1 Phytoremediation of heavy metals: mechanisms, methods and enhancements [DOI]
  2. 2 Animal‐cell culture media: History, characteristics, and current issues [DOI]
  3. 3 Development of botanicals to combat antibiotic resistance [DOI]
  4. 4 History of antimicrobial agents and resistant bacteria.
  5. 5 Antimicrobial effect and pH of chlorhexidine gel and calcium hydroxide alone and associated with other materials [DOI]
  6. 6 Validated microbiological and HPLC methods for the determination of moxifloxacin in pharmaceutical preparations and human plasma [DOI]
  7. 7 Material Requirements for Plastics Used in Medical Devices [DOI]
  8. 8 Antibacterial activity of some medicinal plants of Sri Lanka [DOI]
  9. 9 Microscopic Analysis of Bacterial Inoculum Effect Using Micropatterned Biochip [DOI]
  10. 10 In vitro Anti-inflammatory Activity of Endemic Artocarpus nobilis Thw Found in Sri Lanka [DOI]
  11. 11 Wound healing activity of Celtis timorensis Span. (Cannabaceae) leaf extract in Wistar albino rats
  12. 12 Antibiotics [DOI]
  13. 13 Medicinal and economic plants of the Musaeum Zeylanicum of Paul Hermann.

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

9

Page views

0

PDF downloads

0

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.