Evaluation of Cassava (Manihot esculenta) and Sweet Potato (Ipomea batatas) Starch from South East Nigeria in the Separation of Deoxyribonucleic Acids as Alternative to Agarose Gel
Austine Okpaga Ume, Nkechi Eucharia Egbe, Philip Anthony Vantsawa, Godson Ofobuike Eze, Abdullahi Isyaku Alhaji, Zahra’u Umar, Blessing Timothy
Asian Journal of Biology · pp. 33–45 · Published 10 Sep 2022
10.9734/ajob/2022/v15i430248Abstract
Gel electrophoresis technique is an indispensable tool in biotechnology and among other related fields for separation of nucleic acids and proteins. This study determined the potential of selected cassava and sweet potato starch in the separation of DNA as alternative to agarose gel. The sample pH, gelling temperature and time were determined by Light transmittance method proposed by Craig et al. [1] Standard electrophoresis procedure was used for the starch gel electrophoresis. The result showed that composite starch gelled within 18-21 minutes while agarose and agar-agar gelled after 12 minutes. Cassava starch blended with agar-agar gelled at a temperature of 58oC while sweet potato starch blended with agar-agar gelled between 35oC - 47oC. Agarose and agar-agar maintained 54oC and 53oC respectively. There was no significant difference (P > 0.05) in pH value of the composite starch when compared to 1% agarose gel. Unblended starch samples did not form solid gel except when blended with some amount of agar-agar or agarose powder. Cassava and sweet potato composite starch formed good gel strength at 3% (2.2 g of starch and 0.8 g of agar-agar) but solid gel at 4% (3.6 g of starch and 0.8 g of agar-agar). This study demonstrated the possibilities for agarose cost reduction by 60% when cassava starch (3.6 g) was blended with 0.4 g of agarose. The cassava composite starch (4%) separated DNA molecules comparably to that of 1% agarose. Therefore, the use of these cheaper, accessible and readily available blended starch sources is highly recommended for separation of biomolecules such as DNA.
References (23)
- 1 Edible and Biodegradable Starch Films: A Review [DOI]
- 2 Formation of starch in plant cells [DOI]
- 3 Systematics, Diversity, Genetics, and Evolution of Wild and Cultivated Potatoes [DOI]
- 4 Transfer of test-enhanced learning: Meta-analytic review and synthesis. [DOI]
- 5 Chelating polymer modified P84 nanofiltration (NF) hollow fiber membranes for high efficient heavy metal removal [DOI]
- 6 Structure of the Agarose Constituent of Agar-agar [DOI]
- 7 Introduction to Plant Biotechnology [DOI]
- 8 Modification of starch using low pressure radio frequency air plasma [DOI]
- 9 Chemical Properties of Starch and Its Application in the Food Industry [DOI]
- 10 Physicochemical Properties of Starches Obtained from Three Varieties of Chinese Sweet Potatoes [DOI]
- 11 Improvement of rheological, thermal and functional properties of tapioca starch by using gum arabic [DOI]
- 12 Effect of heat moisture treatment and annealing on physicochemical properties of red sorghum starch [DOI]
- 13 What Is Being Learned About Starch Properties from Multiple‐Level Characterization [DOI]
- 14 Combined effect of fermentation, sun-drying and genotype on breadmaking ability of sour cassava starch [DOI]
- 15 Carbohydrates of the Kernel [DOI]
- 16 Production and quality evaluation of extruded snack from blends of bambara groundnut flour, cassava starch, and corn bran flour [DOI]
- 17 Stabilization of Paste Viscosity of Cassava Starch by Heat Moisture Treatment [DOI]
- 18 Introduction to Agarose and Polyacrylamide Gel Electrophoresis Matrices with Respect to Their Detection Sensitivities [DOI]
- 19 Potential of cassava flour as a gelling agent in media for plant tissue cultures [DOI]
- 20 Cassava: Extraction of starch and utilization of flour in bakery products [DOI]
- 21 Phase Transition of Waxy and Normal Wheat Starch Granules during Gelatinization [DOI]
- 22 Recherches sur l'amidon III. Fractionnement et purification de l'amylose de maïs naturel [DOI]
- 23 Physicochemical Characterization of Cassava (Manihot esculenta) and Potato (Ipomea batatas) Varieties from Southeast of Nigeria in Comparison with Agarose for use in the Separation of Deoxyribonucleic Acid [DOI]
Cited by 0
No indexed citations yet.
Related research
- Plasmid Profile of Multidrug Resistant Bacteria Isolated from Wound Swabs from Hospital Patients in Akure, Nigeria — shares topic coverage
- Evaluation of the Protective Effect of Acacia senegal Extract against di-(2-ethylhexyl phthalate) Induced Hepato- and Neurotoxicity in Rats — shares topic coverage
- Epidemiological and Biological Parameters of Monoclonal Plasma Cell Dyscrasias in Thirty One Patients Consulting a Moroccan Pasteur Institute — shares topic coverage
- Hybrid Approach for Electrophoresis Pattern of Seed Storage Proteins and Soil Study (Pedology); a Study on Intra Specific Diversity of Stachys inflata Benth — shares topic coverage
- Identification of Hemin-binding Protein of Oral Streptococci via Electrophoresis in SDS Polyacrylamide Gel — shares topic coverage
Article metrics
Real usage data collected on this platform.
25
Page views
0
PDF downloads
2
Outbound clicks
0
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.