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Research Article Open access CC BY 4.0

Evaluation of Antifungal Activity of Cow Urine Based Mancozeb Formulation against Fungal Infection Affecting Wheat Seed Germination

Umesh S. Bhoi, Rohit H. Patil, Suvarna K. More, Gautam P. Sadawarte, Jamatsing D. Rajput

Asian Research Journal of Agriculture · pp. 356–367 · Published 26 Dec 2025

10.9734/arja/2025/v18i4796

Abstract

The yield limit in any agricultural system is determined by the seeds. As a result, in recent years, farmers have become more conscious about seed health. Identification of seed-borne pathogens is an important step in seed health and treatment of crop diseases. This research was conducted to prepare and evaluate the effectiveness of a stable cow urine-based mancozeb suspension concentrate formulation against the presence of seed-borne diseases in wheat seeds. The effectiveness of seed treatments with standard mancozeb fungicides and cow urine-based mancozeb formulations in reducing seed-borne fungi was investigated using two methods. The Petri dish technique and the rolled paper towel method were used to assess germination and the presence of fungal infection around the seeds. A dose of 1 g/kg of cow urine-based mancozeb formulation showed equivalent results to the standard mancozeb dose (2.5 g/kg), which significantly reduced the standard dose, which reduced environmental pollution and increased seed germination.

Antifungal activity cow urine mancozeb fungicides fungal infection and seed germination

Cited by 2

Cow Urine’s Agricultural Renaissance: Enhancing Crop Yield and Environmental Sustainability

Umesh S. Bhoi, Rohit H. Patil, Sunil H. Koli · Cureus Journal of Agriculture and Food Science · 2026

Showing 1 of 2 known citations — external sources report more than can currently be individually listed.

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