Evaluation of Anticancer Effect of Aegle marmelos in Human Breast Cancer Cells by In-vitro Analysis

Abstract

Background: Cancer is uncontrolled division and proliferation of abnormal cells in the body. Nowadays, therapeutic Treatment of cancer has become a great clinical challenge and alternative medicines are being extensively studied to cure cancer. Aegle marmelos is one such plant Which has many pharmacological activities. Aim: To study the anticancer activity of Aegle marmelos which promotes cell death in human cancer breast cells MCF7 by modulating wnt/beta catenin expression. Materials and methods: Human Breast Cancer cell line (MCF-7) was purchased from NCCS.Cell viability was assayed using a modified colorimetric technique that is based on the ability of live cells to convert MTT. The specificity of the amplification product was determined by melting curve analysis for each primer pairs. The obtained data were analyzed statistically by one-way analysis of variance (ANOVA) and Duncan's multiple range test with a computer-based software. Results:  the viability of cancer cells after addition of Aegle marmelos has decreased from 100% to 50% with increase in concentration of the extract 100-600 micrograms/ml. It is also evident that there was a fold change in control over Wnt m-RNA expression of MCF7 that decreased significantly on the addition of Aegle marmelos extract. The results also showed that there was a fold change in control of the beta catenin m-RNA expression of MCF7,which decreased significantly in addition to Aegle marmelos. Conclusion: The present study concluded an innovative finding that Aegle marmelos promotes cell death in MCF7 cells by modulating the pathway. The plant extract also possesses hepato- renal protective effect thus, it can be used as a novel and safe anti-cancer drug against breast cancer cells.