Evaluating the Prevalence of Malaria Parasite Infection among Adults in Wetlands Using Nested PCR and High Resolution Melting Analysis
Faith Ajiebabhio Ogbole, Chidi Uzoma Igwe, Henrietta Chinyere Onuoha, Chiamaka Perpetua Nzebude
Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 53–63 · Published 7 Aug 2023
10.9734/ajbgmb/2023/v14i4322Abstract
Background: Wetlands serve as breeding grounds for mosquitoes, the vector of malaria Plasmodium. Effective detection of Plasmodium infection requires a very sensitive method. Aim: The aim of the present study was to evaluate the prevalence of malaria parasite infection in Sagbama LGA, Bayelsa State using nested PCR and high resolution melting analysis (HRMA) technique in comparison with microscopy. Methods: A community based cross sectional study design was employed to randomly select 206 study participants. DNA was extracted from 200 ⴗl of whole blood of each participant and a set of primers was used to target and amplify the 18S rRNA gene of Plasmodium falciparum in both molecular methods. Result: The prevalence of Plasmodium falciparum infection by microscopy was 33.01% (5.8% asymptomatic, 17% mild and 10.2% severe malaria).The prevalence of malaria parasite infection by nested genomic PCR was 71.36% (asymptomatic, 42.20% mild, 18.93%and severe malaria, 10.19%). Prevalence of malaria parasite infection by high resolution melting analysis 92.71% (asymptomatic, 63.59%, mild, 18.93% and severe, 10.19%). All the study participants that were positive for microscopy and nested genomic PCR were also positive for high resolution melting analysis. There were 59.71% and 21.36% false negatives by microscopy and nested PCR respectively. McNemar`s test for pair-wise performance comparisons among the different methods was statistically significant (p < 0.001). High parasite density (19927 ± 749 parasites/µl blood) and hyper parasite density (103667 ± 5214 parasites/ⴗl of blood) were found among asymptomatic and severe malaria subjects (P < 0.000). Conclusion: In conclusion, a high malaria parasite infection prevalence of 92.72% was found in Sagbama LGA. This requires urgent attention especially asymptomatic malaria. HRMA was the most sensitive molecular method and is therefore recommended for future molecular detection of malaria infection.
References (21)
- 1 How to Calculate Sample Size for Different Study Designs in Medical Research? [DOI]
- 2 Prevalence, Parasite Density and Determinants of Falciparum Malaria Among Febrile Children in Some Peri-Urban Communities in Southwestern Nigeria: A Cross-Sectional Study [DOI]
- 3 Prevalence of malaria parasites in adults and its determinants in malaria endemic area of Kisumu County, Kenya [DOI]
- 4 Malarial Anaemia and Anaemia Severity in Apparently Healthy Primary School Children in Urban and Rural Settings in the Mount Cameroon Area: Cross Sectional Survey [DOI]
- 5 Development of High Resolution Melting Analysis for the Diagnosis of Human Malaria [DOI]
- 6 An Automated Microscopic Malaria Parasite Detection System Using Digital Image Analysis [DOI]
- 7 Extraction of human genomic DNA from whole blood using a magnetic microsphere method [DOI]
- 8 A molecular survey of febrile cases in malaria-endemic areas along China-Myanmar border in Yunnan province, People’s Republic of China [DOI]
- 9 Comparison of methods for detecting asymptomatic malaria infections in the China–Myanmar border area [DOI]
- 10 High malaria parasitemia among outpatient febrile children in low endemic area, East-Central Tanzania in 2013 [DOI]
- 11 Parasite density in severe malaria in Colombia [DOI]
- 12 Survey of ethnobotanical cocktails commonly used in the treatment of malaria in southwestern Nigeria [DOI]
- 13 Induction of apoptosis by quercetin: different response of human chronic myeloid (K562) and acute lymphoblastic (HSB-2) leukemia cells [DOI]
- 14 Multisensor earth observations to characterize wetlands and malaria epidemiology in Ethiopia [DOI]
- 15 Sensitivity and specificity of 14 SARS-CoV-2 serological assays and their diagnostic potential in RT-PCR negative COVID-19 infections [DOI]
- 16 Association of Undiagnosed Pre-Diabetes and Type-2 Diabetes Mellitus with Interleukin-2 mRNA Expression Among Adults in Bayelsa State, Nigeria [DOI]
- 17 Distribution of Body Mass Index and Abdominal Obesity in Bayelsa State with Associated Interleukin-2 Gene Expression [DOI]
- 18 Upregulation of Interleukin-2 Among Hypertensive Subjects in Bayelsa State, Nigeria [DOI]
- 19 Application of High-Resolution Melting (HRM) Technique Towards the Detection of Asymptomatic Malaria in a Malaria Endemic Area of Southeastern Iran Under Elimination Program [DOI]
- 20 International Journal of Nanomedicine [DOI]
- 21 Molecular and Cellular Biochemistry
Cited by 12
Faith Ajiebabhio Ogbole, Chidi Uzoma Igwe, Henrietta Chinyere Onuoha, Chiamaka Perpetua Nzebude · International Journal of Biochemistry Research & Review · 2023
Faith Ajiebabhio Ogbole, Teke Calmday Enegborienegha · Asian Journal of Research in Biochemistry · 2024
Faith Ajiebabhio Ogbole, Anthony Anwuli Ogbuta, Hilary Izuchukwu Okagbue · BMC Environmental Science · 2024
Faith Ajiebabhio Ogbole, Chidi Uzoma Igwe, Chinyere Henrietta Onuoha · Scientific African · 2024
Showing 4 of 12 known citations — external sources report more than can currently be individually listed.
Related research
- ABO Blood Groups and Malaria Prevalence in a Referral Hospital in Nigeria — shares topic coverage
- Prevalence of Malaria among Pregnant Women and Children Under Five Years in Abi Local Government Area, Cross River State, Nigeria — shares topic coverage
- A Study on Relationship of Thrombocytopenia and Likelihood of Severe Malaria in Patients Admitted With Malaria in a Tertiary Care Hospital of Dakshina Kannada — shares topic coverage
- Platelet Alteration in Plasmodium vivax Malaria Patients in Thailand — shares topic coverage
- Patients’ Perception of Health Care Services in Malaria Endemic Area in Mali: Villages with Health Care Services Versus villages Without Health Care Services — shares topic coverage
Article metrics
Real usage data collected on this platform.
57
Page views
0
PDF downloads
32
Outbound clicks
12
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.