Preventive Effects of Resveratrol against Beta Adrenergic Catecholamine –Induced Acute Myocardial Stress during Experimental Hyperglycemia in in vivo and in vitro Models

Abstract

Aim: To evaluate the cardioprotective effects of resveratrol during hyperglycemic conditions in in vitro and in vivo models. Study Design: H9c2 cardiomyocyte cells were used as in vitro models and adult male Wistar strain albino rats were used as in vivo models. Activities of LDH and levels of lipid peroxides, total reduced glutathione were the end point indicators for the in vitro studies.  For the in vivo studies the activities of membrane bound ATPases, levels of lipid peroxides, enzymic and non enzymic antioxidants were the end point indicators.  Place and Duration of Study: The study was conducted at the Department of Microbiology and Biotechnology, Bangalore University, Bangalore, between January 2014 and June 2014. Methodology: To mimic myocardial injury during diabetic conditions (in vitro), the H9c2 cells were maintained in high glucose environment followed by isoproterenol challenge. For in vivo studies the animals were segregated as follows: Untreated control; Myocardial stress induced animals (Isoproterenol 150 mg/kg body wt i.p); Diabetic rats (Streptozotocin, 50 mg/kg body wt,i.p);  Myocardial stress induced diabetic rats; resveratrol per se (5 mg/kg.body wt. orally for 21 days), and resveratrol pretreated prior to induction of diabetes and myocardial stress. Results: H9c2 cells given glucose insult and challenged with isoproterenol showed severe cytotoxicity and stress as elicited by increased LDH release, increased lipid peroxides and depleted GSH levels. These changes were prevented in the cells pretreated with resveratrol prior to isoproterenol/glucose challenge. The diabetic rats induced with myocardial stress showed significant alterations in the activity of membrane bound phosphatases, levels of lipid peroxides, enzymic and non enzymic antioxidants. Pre-treatment with resveratrol prevented these alterations thereby implicating cardioprotective effects during hyperglycemic conditions Conclusion: Resveratrol could combat myocardial stress during experimental hyperglycemia in in vitro and in vivo models.