Premna odorata Volatile Oil as a New Mycobacterium tuberculosis Growth Inhibitor  for the Control of Tuberculosis Disease

Abeer H. Elmaidomy; Hossam M. Hassan; Elham Amin; Waleed Mohamed; Mona H. Hetta

doi:10.9734/EJMP/2017/38375

Research Article Open access CC BY 4.0

Premna odorata Volatile Oil as a New Mycobacterium tuberculosis Growth Inhibitor for the Control of Tuberculosis Disease

Abeer H. Elmaidomy, Hossam M. Hassan, Elham Amin, Waleed Mohamed, Mona H. Hetta

European Journal of Medicinal Plants · pp. 1–11 · Published 22 Dec 2017

10.9734/EJMP/2017/38375

Abstract

Aims: This study aimed to identify and compare Premna odorata Blanco volatile oil (VO) for the first time; isolated from different plant organs (leaves, young stems, and flowers) with evaluating the oil antituberculosis (anti-TB) activity. Study Design: Experimental design was carried out by using hydrodistillation method, GC/MS analysis and MeDipro Mycobacterium tuberculosis (MTB) Antigen ELISA Technique (MMA-ELISA) accompanied by polymerase chain reaction (PCR) analysis. Place and Duration of Study: This study was carried out at Faculty of Pharmacy, Beni-Suef University, between May to July 2017. Methodology: P. odorata VO was identified using GC/MS analysis, the oil anti-TB activity was evaluated using in vitro and in vivo MMA-ELISA accompanied by PCR analysis. Results: GC/MS analysis revealed that P. odorata VO consisted of monoterpenes, sesquiterpenes, diterpenes and higher alkanes; where monoterpenes and sesquiterpenes were represented the major oils fractions. Trans-caryophyllene (29.403% & 14.638%) and β-phellandrene (22.390% & 11.701%) were the major compounds in the leaves and young stems oils, respectively. While α-pinene (38.160%) was a characteristic component of the flower's oil. MMA-ELISA showed that a dose of 100 µl/ml in vitro and 300 µl/ml in vivo; the leaves, young stems, and the flowers oils separately had significant anti-TB activities with measured values > 1.5 µg/ml MTB antigen; while the three organs oils in combination 1:1:1 increased the potency of the oils against MTB with measured values < 1.5 µg/ml MTB antigen with PCR negative analysis. Conclusion: P. odorata VO exhibited Anti-TB activity which Anti-TB could be related to the presence of cyclic terpenes (major) and acyclic oxygenated terpenes (minor) compounds.

Premna odorata GC/MS trans-caryophyllene β-phellandrene α-pinene anti-TB

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Abeer H. Elmaidomy, Hossam M. Hassan, Elham Amin, Waleed Mohamed, Mona H. Hetta (2017). Premna odorata Volatile Oil as a New Mycobacterium tuberculosis Growth Inhibitor for the Control of Tuberculosis Disease. European Journal of Medicinal Plants , 1–11. https://doi.org/10.9734/EJMP/2017/38375

Affiliations

Abeer H. Elmaidomy — Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, 62514, Egypt
Hossam M. Hassan — Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, 62514, Egypt
Elham Amin — Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, 62514, Egypt
Waleed Mohamed — Department of Biochemistry, Kasr El-Eini Teaching Hospital, Cairo University, Egypt
Mona H. Hetta — Department of Pharmacognosy, Faculty of Pharmacy, Fayoum University, 63514, Egypt

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