Survey and Identification of Bacteria and Fungi Associated with Two Dumpsites in Akure Metropolis

Abstract

This study was conducted to survey and identify bacteria and fungi associated withb two major dumpsites in Akure metropolis from the months of April, June and August, 2014.     Soil samples were taken from the two waste-dump sites at different intervals. A random sample of the two soils were taken into two different clean nylon bags and taken for culture on sterile and freshly prepared Nutrient Agar (NA) and Potato Dextrose Agar (PDA). All techniques were carried-out under manufacturers’ instructions standard laboratory conditions. The inoculation was done in triplicate and recorded as average total  viable bacteria and fungi in the soil sample.Nine (9) different bacteria and fungi were isolated, characterised and identified from the two dumpsites. The bacteria identified were Staphylococcus aureus, Serratia marcescens, Klebsiella sp, Streptococcus faecalis, Bacillus subtilis, Escherichia coli, Micrococcus luteus, Proteus vulgaris and P. mirabilis while the fungi identified were Aspergillus flavus, A. fumigatus, A. niger, Fusarium moniliformis, Mucor mucedo, Candida albican, Rhizopus stolonifer, Neurospora sp. and Penicillium digitatum of all the organisms isolated during the period of survey, S. aureus had the highest occurrence of 32.00 cfu and 24.25 cfu in Igbatoro and Fiwasaye dumpsite respectively while Neurospora sp recorded highest occurrence of 5.50 sfu in Igbatoro and A. niger recorded highest occurrence of 4.25 sfu at Fiwasaye dumpsite. All the organisms isolated are suggestive of the public health hazard posed by the study areas.