Enzymatic Activity of Rhizobacillus Isolated from Tomato Rhizosphere
K. J. Ayantola, E. D. Fagbohun
Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 11–19 · Published 16 Jul 2020
10.9734/ajbgmb/2020/v4i330106Abstract
Aim: The aim of this study was to isolate Rhizobacillus from tomato rhizosphere and its screening for the production of hydrolytic enzymes to be used as a biocontrol agent. Place of Study: The study was carried out in the Department of Microbiology, Faculty of Science, Ekiti State University, Ado Ekiti, Nigeria in August 2018. Methodology: Isolation was of Rhizobacillus carried out from rhizospheric soil of tomato plant at agro-zone along Afao road Ado Ekiti using pour plate method. Enzyme assay was carried out on the bacterial isolates, to examine their ability to produce hydrolytic enzyme required for biocontrol of phytopathogenic fungi. Isolates were tentatively identified with the help of Bergy’s Manual of Systemic Bacteriology. Results: Ten strains were tentatively identified as B. brevis, B. circulans BC1, B. macquariensis BM1, B. macquariensis BM2, B. macerans, B. macquarensis BM3, B. alcalophilus B. macerans BC11, B. circulans BC3 and B. macerans BC9. All the Isolates demonstrated the ability to produce hydrolytic enzymes with the highest activity recorded in Bacillus macquariensis BM2 (60.28 µmol) for chitinase, Bacillus macerans BC9 (11.14 µmol) for Protease, Bacillus macquariensis BM2 (150.00 µmol) for Glucanase, and Bacillus circulans BC1 (46.45 µmol) for cellulase respectively. In conclusion the Bacillus strains isolated from rhizosphere are promising and could be used in bioprocessing technology to produce hydrolytic enzymes for the purpose of biocontrol in management of phytopathogenic fungi.
References (12)
- 1 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar [DOI]
- 2 Plant growth promoting rhizobacteria as biofertilizers [DOI]
- 3 The Hippo Signaling Pathway Coordinately Regulates Cell Proliferation and Apoptosis by Inactivating Yorkie, the Drosophila Homolog of YAP [DOI]
- 4 Plant growth-promoting rhizobacteria and root system functioning [DOI]
- 5 Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani [DOI]
- 6 Hormonal interplay during adventitious root formation in flooded tomato plants [DOI]
- 7 Biological control of soilborne plant pathogens by a β-1,3 glucanase-producing Pseudomonas cepacia [DOI]
- 8 Enrichment of chitinolytic microorganisms: isolation and characterization of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel Streptomyces strain [DOI]
- 9 Production of an antifungal protein for control of Colletotrichum lagenarium by Bacillus amyloliquefaciens MET0908 [DOI]
- 10 Production of fungal cell wall degrading enzymes by a biocontrol strain of Bacillus subtilis AF 1.
- 11 Enzymatic hydrolysis of chitin by Myrothecium verrucaria chitinase complex and its utilization to produce SCP. [DOI]
- 12 Host-endophyte-pest interactions of endophytic Fusarium oxysporum antagonistic to Radopholus similis in banana (Musa spp.)
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