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Research Article Open access CC BY 4.0

Enhanced in vitro Seedling Recovery in Nothapodytes nimmoniana (J. Graham) Mabberly

Tasiu Isah, Abdul Mujib

Biotechnology Journal International · pp. 35–42 · Published 26 Jan 2015

10.9734/BBJ/2015/15368

Abstract

Enhanced in vitro Seedling Recovery in Nothapodytes nimmoniana (J. Graham) Mabberly Nothapodytes nimmoniana is an endangered medicinal tree and regarded most convenient natural source for large scale isolation of anticancer alkaloid camptothecin. In order to meet pharmaceutical market demand for camptothecin and conserve natural population of the species, in vitro culture techniques can be employed for mass propagation and production of the alkaloid. Seed germination is one of the difficult challenges in the establishment of the aseptic in vitro cultures. Pre-germination treatments of soaking in water, GA3 solution and aseptic excision of the embryo axis were employed to enhance seed germination and seedling recovery respectively for further in vitro studies. Seed soaking in water or GA3 solution for 24 hours enhanced the germination capacity with better response obtained when seeds were soaked in GA3 (2.6 µM) solution and germinated on half strength MS medium supplemented with the GA3. Removal of the outer, inner seed coat and inoculation of the embryo axis on medium amended with or without GA3 reduced seedling recovery time to 3-4 weeks. Seedlings obtained on medium fortified with GA3 showed vigorous growth with complete plantlet development than on PGR-free medium. The strategy can be employed to reduce seedling recovery time to within 3-4 weeks compared to 7-10 weeks required for germinating the seeds in vitro.

Nothapodytes nimmoniana seed germination pre-germination treatment in vitro propagation conservation camptothecin

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Cited by 19

Biomass and camptothecin production in the calcium chloride elicited and liquid medium overlayed Nothapodytes nimmoniana (J. Graham) Mabberly callus cultures

Tasiu Isah, Samiya Masood, Shahid Umar · Vegetos · 2021

Proteome study of embryogenic competence acquisition in the callus cultures of Nothapodytes nimmoniana (J. Graham) Mabberly

Tasiu Isah, Shahid Umar · Acta Physiologiae Plantarum · 2019

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