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Research Article Open access CC BY 4.0

Emodin Isolation from the Leaves of Pteridium acquilinum

M. E.. Khan, E. T. Williams, A. Abel

Current Journal of Applied Science and Technology · pp. 1–6 · Published 22 Mar 2019

10.9734/cjast/2019/v33i630106

Abstract

This study was designed to isolate and characterize the active compound(s) from the leaf extract of Pteridiumacquilinum after the aqueous and methanolic leaf extracts had been investigated on some female rats hormones One anthraquinoneemodin (1, 3, 8-trihydroxy-6-methyl-anthra-quinone) was successfully purified from the methanolic extract of the medicinal plant by Chromatography (VLC, TLC and Sephadex). The initial elution was with n hexane– ethyl acetate–methanol (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin. The Vacuum Liquid Chromatography (VLC), fraction (from the methanol extract) was analyzed by Nuclear magnetic resonance (NMR) and the chemical structure of the anthraquinone was confirmed by 1H-NMR and 13C-NMR analyses. This is the first time this anthraquinone, emodin is being reported from P. aquilinum.

P. aquilinum anthraquinones vacuum liquid chromatography (VLC) emodin

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