Occurrence, Distribution and Antibiotic Sensitivity Pattern of Bacteria Isolated from Some Selected Fishes Sold in Bodija Market, Ibadan, Nigeria

Abstract

Fish constitutes the cheapest source of animal protein in the world. The nutritional qualities and the shelf ability of fish can be affected due to microbial contamination after harvesting. Hence, this study is designed to investigate the microbiological analysis of fish and the antibiotic sensitivity pattern of the isolated bacteria from some selected fish sold in Bodija Market in Ibadan, Nigeria. Three different fish samples: Catfish (Clarias gariepinus), Sabalo fish (Prochilodus lineatus) and Tadpole fish (Raniceps raninus) were bought from Bodija market in an ice packed bag and then transported to Microbiology laboratory for further analyses. Microbial analysis were carried out using serial dilution and pour plate methods. The antibiotic sensitivity pattern on the bacteria isolates were carried out using commercial antibiotics. E. coli, Pseudomonas aureginosa, Staphylococcus aureus, Serratia spp, Klebsiella spp, Salmonella spp, Shigella spp were isolated. Gram negative bacteria were more susceptible to most of the antibiotics used than gram positive bacteria. E. coli showed the highest susceptibility to all the antibiotics with varied zone of inhibitions. Salmonella spp show the highest susceptibility to septrin with the lowest zone of inhibition to pefloxacin. Shigella spp had the lowest zone of inhibition to gentamycin, rocephin, septrin and erythromycin when compared to other bacterial isolates. Serratia spp exhibited highest zone of inhibition to gentamycin. Fish contamination can be averted by employing good environmental conditions and preservation methods to avoid spoilage. The antibiotics sensitivity showed that all the bacteria isolated were more susceptible to antibiotics, thereby developing a resistant strain.