Potentiality of Garlic and/or Green Tea Aqueous Extracts in Attenuating Pesticides Induced Toxicity in Albino Rats

Abstract

Aim: The present work aimed to evaluate the effect of each of green tea (GT) and garlic (Ga) aqueous extract and their combination on pesticide Duracine induced changes in plasma levels of some indices of oxidative stress; and in protein electrophoretic patterns, in albino rats. Study Design: Forty rats were divided into 8 groups 5rats each and treated orally for 14 days as follows: G1 (control);-G 2 (200mg GT per animal);- G 3 (1ml of 50% of garlic):- G4 (200mg green tea per animal and 1ml of 50% of garlic); G 5 20mg /Kg bwt [equivelant 1/14 LD50 of Duracine] ,G6 (green tea and Duracine):- G7 (garlic and Duracine):- G8 (green tea, garlic and Duracine). Plasma activities of acetylcholinesterase (AChE), glutathione-S-transferase (GST) and glutathione reductase (GR) activities and levels of malondialdehyde (MDA) and total reduced glutathione (GSH) were analyzed using standard methods. Results: Duracine intoxication induced significant decrease in plasma AChE, GST and GR. It elevated plasma MDA, decreased plasma total GSH, as well as, changes in plasma protein electrophoretic pattern. Individual supplementation with GT induced slight improvement in AchE, GSH and MDA. On the other hand, supplementation with aqueous extract of garlic induced significant (P<0.05) enhancement in the level of GSH in the intoxicated animals. Combined supplementation with GT+Ga produced significant (P<0.05) counteracting effect in MDA,GSH,GST and GR, as well as ,counteracting changes in plasma protein pattern was not remarkable this may be due to plasma protein half life. Conclusion: It seems that, combined supplementation of green tea and garlic improve the oxidative stress parameters but needa more time to improve other toxicity markers.