Effects of Seed Invigoration Treatments with Bio-priming on Germination, Vigour and Seedling Growth in Black Gram (Vigna mungo L.)

Abstract

A study had been conducted in black gram to observe the effect of different seed invigoration treatments with bio-priming for seed germination, seedling growing and vigour. The variety of black gram i.e., Uttara, was selected and seed had been treated with control (T1); Seeds soaked in distilled water/ hydropriming (T2); Rhizobium leguminosarum 10% (T3); Rhizobium leguminosarum 15% (T4); Rhizobium leguminosarum 20% (T5); Rhizobium leguminosarum 25% (T6); Rhizobium leguminosarum 30% (T7) respectively. Treated seeds were grown in different glass plate and patri plate at Seed Testing Laboratory, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, India. Seed invigoration with Rhizobium leguminosarum at 20% followed by T4 was found very effective for seed germination with vigorous seedlings. Higher seed germination percentage (82.60), shoot length (17.89 cm), root length (12.99 cm), seedling fresh weight (1.87 g), seedling dry weight (0.19 g), seedling vigour index I (2393.62) and seedling vigour index II (15.96) results were observed for the seed bioprimed with Rhizobium leguminosarum at 20%. Seed treatment with Rhizobium leguminosarum at 20% recorded better performance than rest the treatments for all characters observed. The present investigation clearly depicted that the germination, vigour and seedling growth revalidated seed lots can be improved by pre-sowing and invigoration treatments.