Effects of Micro Current, Collagen, and DMAE on the Connective Tissue of Wistar Rats Evaluated by Methods Histological and Gravimetric

Abstract

Introduction: Skin aging is a degenerative process, involving intrinsic and extrinsic factors, being related to photoaging, oxidative stress and inflammatory response, a process that induces the degenerative effect in the collagen network. In order to minimize skin aging, the intake of collagen, micro current and DMAE is an alternative in the aesthetic environment, however, the mechanisms of action involved in these phenomena are not fully understood. Objective: To evaluate the relationship between CM actions and direct collagen administration such as oral supplementation and DMAE, in the effectiveness or efficiency in the rejuvenation of integrodermal tissues in rats that did not suffer tissue injury, from their observed effects on connective tissue. Materials and Methods: The sample consisted of 12 Wistar rats (Rattus norvegicus), subdivided into four groups, respectively, control group (CG) that will not receive application of micro current (CM); treatment-7 group (GT7), which will receive an MC applications for one week once a day; collagen treatment group (GTCo), in which hydrolyzed collagen will be administered orally for 1 week (1 per day); and DMAE treatment group (GTD), in which intradermal microinjection of DMAE will be applied. After treatment, the skin tissues of the animals were analyzed by histological techniques and by gravimetric method. Results: The GTCo presented an average area in μm2 and a higher percentage when compared to the control by analysis of variance with p: 0.044. Conclusion: The use of oral collagen for seven days in animals was efficient after histological analysis.