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Research Article Open access CC BY 4.0

Effects of Foliar Application of Brassinolide and Humic Acid on Protein and Nutrient Uptake of Black Gram (Vigna mungo L.)

Kiran N More, Onkar R. Hirwe, Apeksha M Suryawanshi, Shital S. Patil, Shrikrishna B. Narale, Suraj N More, Harshraj Salunkhe, Anita Jaswal

International Journal of Plant & Soil Science · pp. 428–441 · Published 26 Nov 2025

10.9734/ijpss/2025/v37i115856

Abstract

The field experiment was conducted during the Kharif season of 2022-2023 at the Lovely Professional University, Jalandhar, Punjab. To study the effect of brassinolide and humic acid on growth and yield of black gram. The experiment was laid out in a Randomized Block Design (RBD) having three replication and eight treatments by using MASH-114 variety. The treatments were: T0-Control T1-(100% RDF), T2-RDF + Humic acid 20kg ha-1 (Soil applied), T3-RDF + Humic acid 0.1% (Foliar applied), T4-RDF + Brassinolide 0.25ppm (Soil applied), T5-RDF + Brassinolide 0.1ppm (Foliar applied), T6-RDF + Humic acid 20kg ha-1 + Brassinolide 0.25ppm (Soil applied), T7-RDF + Humic acid 0.1%+ Brassinolide 0.1ppm (Foliar applied) were used for this study. According to the findings of a field investigation the protein and nutrient uptake black gram were considerably impact due to application of humic acid and brassinolide. The application of humic acid considerably enhanced the content and uptake of NPK as compared to the other treatments. Available NPK, uptake of NPK, content of NPK in plant after harvest of crop were increased significantly due to Humic acid 20kg ha-1 +Brassinolide 0.25ppm Soil applied along with RDF and foliar application of Humic acid 0.1% + Brassinolide 0.1ppm respectively along with RDF in black gram. Humic acid and brassinolide significantly improved NPK content, uptake, and overall performance of black gram, with combined soil and foliar applications being most effective. Future studies should optimize doses, test across locations, and assess long-term soil and crop benefits to enhance nutrient efficiency and sustainable pulse production.

Protein sustainability brassinolide humic acid black gram

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