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Effect of Oral Administration of Methanolic Extract of Ocimum gratissimum on Intestinal Ischemia-Reperfusion Injury in Rats

J. F. Akinrinmade, A. S. Akinrinde

European Journal of Medicinal Plants · pp. 591–602 · Published 27 Aug 2013

10.9734/EJMP/2013/5425

Abstract

Aim: The effect of the methanolic extract of Ocimum gratisimum (OG) leaves on the tissue damage induced by ischemia-reperfusion (IR) injury in the rat intestine was investigated. Study Design: Randomized controlled experiment. Place and Duration of Study: Experimental Animal Unit and Faculty of Veterinary Medicine, University of Ibadan, Nigeria from March to May, 2013. Methodology: 18 rats were divided randomly into 3 groups of 6 rats each. Group I served as control; Group II had IR injury by laparotomy with clamping of the Superior mesenteric artery (SMA) for 30 minutes followed by removal of the clamp for 45 minutes; Group III was pre-treated orally for 7days with methanolic extract of Ocimum gratissimum (MEOG) followed by IR injury. Sections of the duodenum and ileum were cut for histopathological examination. The remaining tissues were processed for the determination of biochemical markers of oxidative stress including Hydrogen peroxide (H2O2), Malondialdehyde (MDA) and Glutathione concentrations. Results: Mean values of MDA and H2O2 levels were significantly elevated (P=0.004 and P=0.03, respectively) in intestinal tissues following IR compared to control while reduced glutathione (GSH) levels were not significantly altered. OG (500mg/kg) caused significant reduction (P=0.02 and P=0.04) in MDA concentrations and H2O2 generation, respectively compared with the IR group. Histopathological examination revealed erosions and stunting of the villi tips in the duodenum and ileum, with severe mononuclear infiltration at the mucosal surface in the IR group. No visible lesions were observed in the intestine of the control group, with no significant alterations in the intestinal epithelium of the OG-treated rats. Conclusion: The results suggest that OG may provide some protection against intestinal mucosal injury induced by ischemia-reperfusion, through its anti-oxidative effects.  

Ocimum gratissimum ischemia reperfusion intestine antioxidant rats

References (35)

  1. 1 Improved method for the determination of blood glutathione.
  2. 2 Free radicals, antioxidants, and human disease: where are we now?
  3. 3 [18] Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides [DOI]
  4. 4 Antioxidant properties of resveratrol: A structure–activity insight [DOI]
  5. 5 Screening of Indian plants for biological activity: I.
  6. 6 Role of xanthine oxidase and granulocytes in ischemia-reperfusion injury [DOI]
  7. 7 Ischemic injury in the cat small intestine: Role of superoxide radicals [DOI]
  8. 8 Ischemia-reperfusion injury: role of oxygen-derived free radicals.
  9. 9 Pathophysiology, Clinical Manifestations, and Prevention of Ischemia-Reperfusion Injury [DOI]
  10. 10 Antioxidant properties of a North American ginseng extract [DOI]
  11. 11 Ischemia/Reperfusion Injury [DOI]
  12. 12 Chemoprevention of 2-acetylaminofluorene-induced hepatotoxicity and lipid peroxidation in rats by kolaviron—A Garcinia kola seed extract [DOI]
  13. 13 Reperfusion injury after intestinal ischemia [DOI]
  14. 14 Pathophysiology of mesenteric ischemia/reperfusion: a review [DOI]
  15. 15 Oxidative Stress and Ischemia-Reperfusion Injury in Gastrointestinal Tract and Antioxidant, Protective Agents [DOI]
  16. 16 Ocimum gratissimum: A Review of its Chemical, Pharmacological and Ethnomedicinal Properties [DOI]
  17. 17 Receptor independent stimulatory effect of noradrenaline on Na,K-ATPase in rat brain homogenate [DOI]
  18. 18 Role of Oxygen-Derived Free Radicals in Hemorrhagic Shock-Induced Gastric Lesions in the Rat [DOI]
  19. 19 Effects of n-acetylcysteine in a rat model of ischemia and reperfusion injury [DOI]
  20. 20 Role of Induced Nitric Oxide in the Initiation of the Inflammatory Response After Postischemic Injury [DOI]
  21. 21 Antidiarrhoeal effects of Ocimum gratissimum leaf extract in experimental animals [DOI]
  22. 22 Antioxidant activity, nitric oxide scavenging activity and phenolic contents of Ocimum gratissimum leaf extract [DOI]
  23. 23 Autocrine/Paracrine Secreted Frizzled-related Protein 2 Induces Cellular Resistance to Apoptosis [DOI]
  24. 24 Effect of Zinc-Carnosine Chelate Compound (Z-103), A Novel Antioxidant, on Acute Gastric Mucosal Injury Induced by Ischemia-Reperfusion in Rats [DOI]
  25. 25 Antimutagenic activity of extracts of leaves of four common edible vegetable plants in Nigeria (West Africa) [DOI]
  26. 26 The anthelmintic effects of the leaf extract of Ocimum gratissimum (L.) [DOI]
  27. 27 Effect of natural antioxidant Ocimum gratissimum in modulating neurodegenerative changes in rats fed with high concentration of lead acetate [DOI]
  28. 28 Endotoxemia and Remote Organ Injury Following Intestinal Reperfusion [DOI]
  29. 29 Effect of Ischemia on the Canine Large Bowel: A Comparison with the Small Intestine [DOI]
  30. 30 Inhibition of lipid peroxidation by superoxide dismutase following regional intestinal ischemia and reperfusion [DOI]
  31. 31 Heart energy metabolism after intestinal ischaemia and reperfusion [DOI]
  32. 32 Effect of deferoxamine and L-arginine treatment on lipid peroxidation in an intestinal ischaemia-reperfusion model in rats [DOI]
  33. 33 Translocation of 99mTc labelled bacteria after intestinal ischemia and reperfusion [DOI]
  34. 34 Gastro-Protective Properties of the Leaf Extracts of Ocimum gratissimum L. Against Experimental Ulcers in Rat [DOI]
  35. 35 Ischemia-Reperfusion Injury [DOI]

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