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Research Article Open access CC BY 4.0

Effect of Media and Photoperiod on Embryogenic and Organogenic Callus Induction from Mature Seed in Oryza sativa L.

Danielle Christelle Tinak Ekom, Jean Cyrille Mouen Piau, Haïcha Diko Abba, Oungvan Memena, Rodrigue Simonet Poueme Namegni, Abel Wade

Annual Research & Review in Biology · pp. 20–27 · Published 20 Mar 2024

10.9734/arrb/2024/v39i42071

Abstract

Rice is the staple food for billions of people and a source of income for hundreds of millions. This food security crop production needs to be improved to face the projected demands of the exponentially augmenting world’s population and overcome biotic and abiotic stress in the fields. Optimized in vitro culture protocols are a prerequisite to genetic engineering which appears to be the best potential way to improve the rice plant. In this study, ‘Nerica 3’ and ‘Nerica L36’ seeds were in vitro culture tested into 4 media (m1-4), in dark and photoperiod environments. The percentage of callus induction was calculated and callus weight was recorded. Results show that callus induction was influenced by variety × environment and medium × environment interactions, with a strong influence of the environment used.  ‘Nerica 3’ showed the highest mean (88.25%) callus induction after six weeks of incubation on different media. m1 and m2 media showed greater mean callus induction (more than 86%). Higher callus induction came from m1 (82% and 91%) and m2 (81% and 93%) media, while lower rates came from m3 (73%) and m4 (69%) media for embryogenic and organogenic calli respectively. Culturing the explant in dark environment to produce embryogenic callus, resulted in greater callus weight for ‘Nerica 3’ (1.03 g) and ‘Nerica L36’ (0.79 g). This study is a contribution to the rice plant genetic improvement by proposing protocols for somatic embryogenesis of two Nerica rice varieties.

Rice food security biotechnology somatic embryogenesis callus

References (13)

  1. 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
  2. 2 Nutrient requirements of suspension cultures of soybean root cells [DOI]
  3. 3 Transforming our world: the 2030 Agenda for Sustainable Development [DOI]
  4. 4 Rice Production in Asia: Key to Global Food Security [DOI]
  5. 5 Proline and Glutamine Improve in vitro Callus Induction and Subsequent Shooting in Rice [DOI]
  6. 6 Improvement of efficient in vitro regeneration potential of mature callus induced from Malaysian upland rice seed (Oryza sativa cv. Panderas) [DOI]
  7. 7 Rice Biotechnology : A Need for Developing Countries
  8. 8 Influence of photoperiod on growth, bioactive compounds and antioxidant activity in callus cultures of Basella rubra L. [DOI]
  9. 9 Genome‐wide association study of callus induction variation to explore the callus formation mechanism of rice [DOI]
  10. 10 Introducing Gamborg’s B5, a high-potential medium for isolated microspore culture, and presenting a new MS medium-based protocol for androgenic plant regeneration in eggplant (Solanum melongena L.) [DOI]
  11. 11 Genetic transformation of NERICA, interspecific hybrid rice between Oryza glaberrima and O. sativa, mediated by Agrobacterium tumefaciens [DOI]
  12. 12 Application of Biotechnology to Improving the Nutritional Quality of Rice [DOI]
  13. 13 STUDY OF FACTORS INFLUENCING SOMATIC EMBRYOGENESIS IN RICE (ORYZA SATIVA L.)

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