Effect of Desiccation and Chilling Treatment on Somatic Embryo Development and Germination in Rough Lemon (Citrus jambhiri Lush)
Biotechnology Journal International · pp. 136–148 · Published 13 Dec 2013
10.9734/BBJ/2014/6241Abstract
The goal of this study was to evaluate the effect of desiccation and chilling treatments on somatic embryogenesis of rough lemon (Citrus jambhiri Lush.). Styles were cultured on seven culture media (MS I-MS VII) containing Benzylaminopurine (BAP), Kinetin (KN) and Malt Extract for cell proliferation and somatic embryo development. Cell proliferation was maximum on MS IV media but maximum cultures showing somatic embryogenesis (52.08 %) was observed on MS VII media. Embryogenic callus proliferated on MS VII media was subjected to desiccation and chilling treatment for 24, 48, 72 and 96 hours. Embryogenic callus desiccated for 24 and 48 hours in sterile petriplates showed 58.33 and 56.94 % somatic embryogenesis respectively as compared to undesiccated callus (51.98%). Average number of cotyledonary embryos (6.80/culture) in embryogenic cultures from desiccated callus (48 hrs) was more as compared to untreated callus (2.26/culture). There was significantly less number of abnormal embryos (0.60-0.53/culture) in desiccated callus for 48, 72 and 96 hours as compared to untreated callus (7.20/culture). Chilling treatment also improves the average number of cotyledonary embryos and reduces the abnormal development of embryos. Among all the treatments desiccation of embryogenic callus for 48 hrs proved beneficial for improvement of somatic embryo development and germination.
References (35)
- 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
- 2 Regulation of in vitro somatic embryogenesis with emphasis on to the role of endogenous hormones [DOI]
- 3 Influence of gelling agents on culture medium gel strength, water availability, tissue water potential, and maturation response in embryogenic cultures of Pinus strobus L. [DOI]
- 4 Proteomic analysis of somatic embryogenesis in Valencia sweet orange (Citrus sinensis Osbeck) [DOI]
- 5 Enhanced production and germination of somatic embryos by temporary starvation in tissue cultures of Daucus carota [DOI]
- 6 An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush [DOI]
- 7 Improved somatic embryogenesis in wheat by partial simulation of the in-ovulo oxygen, growth-regulator and desiccation environments [DOI]
- 8 Improvement of somatic embryogenesis and plant recovery in cassava [DOI]
- 9 Partial desiccation of mature embryo-derived calli, a simple treatment that dramatically enhances the regeneration ability of indica rice [DOI]
- 10 Somatic embryogenesis in Citrus SPP.: Carbohydrate stimulation and histodifferentiation [DOI]
- 11 Plant development from microspore-derived embryos in oilseed rape as affected by chilling, desiccation and cotyledon excision [DOI]
- 12 Initiation, maintenance and maturation of somatic embryos from thin apical dome sections in Pinus kesiya (Royle ex. Gord) promoted by partial desiccation and Gellan gum [DOI]
- 13 Somatic embryogenesis and plant regeneration from undeveloped ovules and stigma/style explants of sweet orange navel group [ Citrus sinensis (L.) Osb.] [DOI]
- 14 Somatic embryogenesis and plantlet regeneration in mandarin (Citrus reticulata Blanco) [DOI]
- 15 High efficient transgenic plant regeneration from embryogenic calluses of Citrus sinensis [DOI]
- 16 Elimination of Citrus psorosis virus by somatic embryogenesis from stigma and style cultures [DOI]
- 17 Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium [DOI]
- 18 Improved germination of somatic embryos and plant recovery of European chestnut [DOI]
- 19 Cold-enhanced somatic embryogenesis in Pinus patula is mediated by calcium [DOI]
- 20 In vitro micrografting for production of Indian citrus ringspot virus (ICRSV)-free plants of kinnow mandarin (Citrus nobilis Lour × C. deliciosa Tenora) [DOI]
- 21 In vitro response of encapsulated and non-encapsulated somatic embryos of Kinnow mandarin (Citrus nobilis Lour × C. deliciosa Tenora) [DOI]
- 22 Effect of cold storage on somatic embryogenesis systems ofCamellia [DOI]
- 23 In vitro selection of calli of Citrus jambhiri Lush. for tolerance to culture filtrate of Phytophthora parasitica and their regeneration [DOI]
- 24 Water relations in culture media influence maturation of avocado somatic embryos [DOI]
- 25 Callus sieving is effective in improving synchronization and frequency of somatic embryogenesis in Citrus sinensis [DOI]
- 26 Effect of physical desiccation on plant regeneration efficiency in rice (Oryza sativa L.) variety super basmati [DOI]
- 27 Plant regeneration from undeveloped ovules and embryogenic calli of Citrus: Embryo production, germination, and plant survival [DOI]
- 28 Somatic embryogenesis and plantlet regeneration on calluses derived from seedling explants of ‘Kinnow’ mandarin (Citrus nobilisLour, ×Citrus deliciosaTenora) [DOI]
- 29 Comparison of agar and gum karaya as gelling agent for in vitro regeneration of rough lemon (Citrus jambhiri Lush.) plantlets from nodal explants [DOI]
- 30 Desiccation of callus enhances somatic embryogenesis and subsequent shoot regeneration in sugarcane
- 31 Embryogenesisin vitroof severalCitrusspecies and cultivars [DOI]
- 32 Micropropagation of Citrus spp. by Organogenesis and Somatic Embryogenesis [DOI]
- 33 Cold-enhanced somatic embryogenesis in cell suspension cultures of Astragalus adsurgens Pall.: relationship with exogenous calcium during cold pretreatment [DOI]
- 34 Somatic embryogenesis inAesculus [DOI]
- 35 In vitro production of Indian citrus ringspot virus-free plants of Kinnow mandarin (Citrus nobilis Lour × C. deliciosa Tenora) by ovule culture.
Cited by 6
Mozidul Haque, S. M. Shahinul Islam, Sreeramanan Subramaniam · 3 Biotech · 2017
Samaneh Sadat Maleki, Kourosh Mohammadi, Kong Shu Ji · Plant Cell, Tissue and Organ Culture (PCTOC) · 2018
Showing 2 of 6 known citations — external sources report more than can currently be individually listed.
Related research
- Organoleptic and Morphological Analysis of Citrus Peel Powder — shares topic coverage
- Effect of Gamma Radiation on the Variability of Some Quality Criteria in Sidi Aissa Clementine — shares topic coverage
- Plantlet Regeneration from Unfertilized Ovule of Mandarin (Citrus reticulata) — shares topic coverage
- Performance of ‘Valencia Late’ Sweet Orange (Citrus sinensis) on Different Rootstocks in the Gharb Region (Northwestern Morocco) — shares topic coverage
- Integrating Morphological and Molecular Techniques to Identify Intraspecific Crosses in Citrus — shares topic coverage
Article metrics
Real usage data collected on this platform.
71
Page views
0
PDF downloads
45
Outbound clicks
6
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.