Isolation of Fungal Species and Aflatoxin Detection from Grinded Cereals Sold in Dutsin-Ma Metropolis
Khalifa Jamil Saleh, Z. Theophilus Samuel, Ahmad Bello Salim
South Asian Journal of Research in Microbiology · pp. 36–49 · Published 2 Feb 2023
10.9734/sajrm/2023/v15i1280Abstract
Aflatoxins are the most common and potent mycotoxins produced mainly, by Aspergillus species, often found contaminating cereals. This study was aimed at isolating fungal species and detecting aflatoxins from grinded cereals sold in Dutsin-ma metropolis of Katsina State, Nigeria. Forty (40) cereal samples (Maize, Millet, Sorghum and Wheat), 10 of each were collected randomly from 3 different locations in Dutsin-ma metropolis. Standard microbiological procedures were used to isolate and identify fungal species on Potato Dextrose Agar (PDA) and fungal ATLAS [1,2] respectively. The predominant fungi isolated were species of Aspergillus, Mucor and Fusarium. Aspergillus niger had the highest incidence of 32.1%, Aspergillus flavus had 20.2%, followed by Mucor spp. with 13.1%, Aspergillus fumigatus with 11.9%, Aspergillus parasiticus had 8.3%, then Aspergillus lentulus and Fusarium oxysporum had 7.14% each. Presence of Aflatoxins were tested (out of the four aflatoxin types) in the samples using High-Performance Liquid Chromatography (H.P.L.C) (UV-VIS detector) by comparing the retention time of standards to the time on the peaks obtained from the samples during the HPLC analysis. Aflatoxins were detected in all three samples that were tested with the maize sample having the highest % height of B1 (48.10%), Millet had the highest % height of G1 (29.88%). G2 was not detected on the millet samples. All the other 2 samples had all three aflatoxins detected. Percentage height of aflatoxin peaks ranged from 16.60% – 48.10. This study revealed high contamination of grinded cereals by species of Aspergillus, Mucor and Fusarium. Aflatoxins B1, G1 and G2 were also detected, which makes their consumption a public health risk. Proper pre-harvest and post-harvest management of cereals coupled with proper cooking of cereal products before eating are recommended so as to reduce contamination and prevent possible intake of these toxins.
Cited by 0
No indexed citations yet.
Related research
- Aflatoxins, Dairy Products Contamination in the Incidence and Development of Diseases in Children Population – A Short Systematic Review — shares topic coverage
- Differential Expression Pattern of Heat Shock Protein Genes in Toxigenic and Atoxigenic Isolate of Aspergillus flavus — shares topic coverage
- Phenotypic Characterization of Toxigenic Fungi, Evaluation and Effect of Post Treatment on Aflatoxin and Ochratoxin A Content in Smoked Fish and Smoked Bush Meat — shares topic coverage
- Molecular Screening of Fungal Isolates of Palm Oil from South Eastern Nigeria for Aflatoxin and Ochratoxin Biosynthetic Genes Using Multiplex Polymerase Chain Reaction (mPCR) — shares topic coverage
- Determination of Knowledge Based on Aflatoxin Poisoning among Broiler Farmers in Nairobi City County, Kenya — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
0
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.