Physiological Dynamics of Platelets’ Activity in Aged Rats

Abstract

The process of aging inevitably touches the system of hemostasis and leads very often to the development of thrombophilia. Platelets are an important component of hemostasis as they mostly determine its activity on the whole. It’s very interesting to estimate the dynamics of platelet activity in rats as they are important objects of laboratory investigations in the period of their bodies’ evident aging – at the age between 18 and 30 months of life. The aim of the study is to establish the peculiarities of age-specific dynamics of platelet activity in aged rats. There were examined 95 healthy Wister male-rats, including 32 rats at the age of 18 months, 29 rats at the age of 24 months and 34 rats at the age of 30 months. The control group was composed of 27 healthy male-rats of Wistar line at the age of 6 months. We applied biochemical, hematological and statistical methods of investigation. Between 18-30 months of life the rats were noted to have rise of acylhydroperoxides’ content in plasma on 19.9%, thiobarbituric acid-active products – on 18.9% and lowering of plasma antioxidant activity on 23.8%. Between 18-30 months of life healthy rats were found to have gradual acceleration of platelet aggregation (platelet aggregation with ADP at the age of 18 months was equal to 38.4±0.12s, at the age of 30 months – 33.1±0.16s), content increase of platelets’ active forms in their blood (disco-echinocytes at the age of 18 months – 14.0±0.18%, at the age of 30 months – 17.5±0.17%) and number increase of their freely circulating aggregates (small aggregates at the age of 18 months – 3.5±0.09, at the age of 30 months – 7.2±0.13 on 100 freely lying platelets). Found increase of platelet activity in rats contributes greatly to their rising with age morbid aggravation, increase of their bodies’ sensitivity to negative impacts of environmental factors and promotes the realization of hereditary predisposition to different pathology. Received results can be useful for future researches connected with formation aspects of age-specific thrombophilia and can be taken into account at estimation of different variants of life’s experimental prolongation.