Physiological Dynamics of Spontaneous Erythrocytes’ Aggregation of Rats at Last Ontogenesis

Abstract

In modern biology we still have the actual demand of further investigation of aging aspects of mammals and human beings. Great attention in this investigation is devoted to different aspects of blood rheological peculiarities and its regular elements, i.e. in aging aspect, in normal state and in some pathology conditions and, also, on the background of many variants of correctional impacts on an organism. The purpose is to establish the age dynamics of microrheological particular properties of aging rats’ red blood cells. The study involved 95 healthy Wistar male rats that included 32 rats of 18 months of age, 29 rats of 24 months and 34 rats of 30 months of age. We used biochemical, hematological and statistical methods. The control group was represented by 27 healthy Wistar male rats. Examined animals on the background of aging were noted to have activity increase of freely radical lipids’ oxidation in the liquid part of blood at the decrease of AOA from 30.70±0.32% at 18 months to 24.40±0.29% at 30 months. Analogical values in the control group were 1.440±0.007 D233/1 ml, 3.460±0.016 umol/l and 34.8±0.010%, correspondingly. Similar picture of POL dynamics of examined animals was also found in erythrocytes: Levels of AHP and MDA in them gradually increased – from 18 months of life to 30 months of life on 27.2% and 26.1%, correspondingly. At the same time experimental rats between 18 and 30 months of life were noted to have increase of erythrocytes’ aggregation activity with the rise of their summary inclusion into aggregates and quantity of aggregates at the lowering of free erythrocytes’ number (228.70±0.31), in comparison with control rats. Found increase of their aggregation is mostly provided by appearing changes in the charge of erythrocyte membranes due to degradation of some negatively charged glycoproteins on them under the influence of increasing with age lipid peroxidation. Aggregation also increases due to inhibition of adenylate cyclase in erythrocytes what leads to the amount decrease of cyclical adenosine phosphate in them and to the stimulation of Ca2+ inflow into them. Development of oxidative damage in plasma globular protein “bridges” between erythrocytes, providing their aggregation, increases erythrocytes’ disaggregation threshold due to the increase of their connection in aggregates. Age increasing erythrocytes’ aggregation in rats negatively influences microcirculation and contributes significantly to marked with aging morbid aggravation due to strengthening a body’s sensitivity to negative impacts of environmental factors which promote the realization of hereditary predisposition to various diseases.