Helium-Neon Laser Effects on Human Whole Blood by Spectroscopy In vitro Study

Abstract

Low-power helium-neon laser recently has been used numerously in medical applications. FTIR and UV-Vis spectroscopic technique is employed to study the spectral differences in the serum of whole blood samples. Aims: To study (He-Ne) laser (λ= 632 nm, power=2 mW) effect on human whole blood, after irradiated to different times from 10 min to 50 min. Study Design: Human Whole Blood Irradiated to   (He-Ne) laser (λ= 632 nm, power=2 mW).  Place and Duration of Study: Institute of Laser, Sudan University of science and technology (SUST), Soba Hospital, Khartoum- Sudan, February 2018. Methodology: Blood samples were collected from healthy volunteers; blood sample exposed to (H-N) laser and control compared; UV-Vis spectrophotometer and FTIR were used to study the effect of laser radiation. Results: Absorption spectrum and FTIR spectra of whole blood are compared before and after He-Ne laser radiation shows, a significant decrease in intensity. FTIR spectrum of non exposed blood showed the peaks due to O-H (free group), C=O (amide I group), N=O (nitro group), and C-H (aromatic group). N-H (Amino acid (amide II) Laser radiation changes in transmittance in FTIR spectra for C=O group and O-H, N=O, the percentage of transmittance were increased. The most effects are found when whole blood irradiated to He-Ne laser radiation for 10 and 20 min and transmittance decreases for C-H, and N-H, due to denaturation of the protein. Conclusion: Photodegradation of blood components due to absorption of laser radiation causes changes in the structure and conformational changes in the polypeptide and decrease intensity.