Hydroethanolic Extract of Buchholzia coriacea Seeds Alleviates LPS Induced Liver Injury in Rat via Antioxidant and Anti-inflammatory Actions

Abstract

Aim: This study evaluates the hepatoprotective effects of hydroethanolic extract of (defatted) Buchholzia coriacea seed (HEBCS) against lipopolysaccharide (LPS) induced inflammatory liver injury in the rat. Method: Thirty male albino rats (120–130 g) were assigned into five groups (n=6/ group). Group I (Control) and II (LPS) received distilled water orally (p.o.) for seven days while animals in groups III, IV and V were administered HEBCS at 125, 250 and 500 mg/kg body weight (b.w.) p.o. daily for 7 days. LPS was injected on the 7th day at a single dose of 4 mg/kg intraperitoneally (i.p.) to the rats in group II, and groups III, IV and V. Six hours after LPS injection, blood was collected to prepare serum, and liver was removed for preparation of homogenate, histopathology and immunofluorescence staining. Biomarkers of liver function (ALT, AST and ALP) and oxidative stress (NO, SOD, CAT and MDA) were assessed. Pro-inflammatory cytokine (TNF-α) and inducible protein (C-reactive protein) were also assessed. Results: Results showed significant amelioration of liver function markers and levels of pro-inflammatory proteins by HEBCS. Histopathological studies showed a reduction in inflammatory cells and improvement in liver structure in animals treated with HEBCS. Conclusion: Data from the current study show that HEBCS possesses hepatoprotective effects against LPS induced inflammatory liver injury.