Skip to content
Research Article Open access CC BY 4.0

DSPC Liposomes Improve Transport of L-cysteine and Reduce Metabolic Activity

Ramiro M. Perrotta, M. Jimena Prieto, Silvia del V. Alonso, Nadia S. Chiaramoni

Biotechnology Journal International · pp. 1–11 · Published 26 Mar 2016

10.9734/BBJ/2016/24723

Abstract

Aims: In this work, we developed and characterized liposomal formulations that encapsulate L-cysteine to study their further application in drug delivery and amino acid supplementation. The lipids used were 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Methodology: Encapsulation efficiency and amino acid release were determined. For biophysical characterization of the three formulations, the size, surface charge and surface packing were also studied. Cell viability was analyzed with MTT reagent after treatments with formulations ir order to study efficiency of these systems in induce changes in metabolism. Results: Results showed that L-cysteine interacts at the polar head level and that this interaction stabilizes the surface charge and prevents aggregation. We also determined the influence on cell metabolism in all formulations. The presence of L-cysteine in the DSPC formulation induced deeper changes in metabolism, evidencing that this formulation provides better transport of this amino acid. Conclusion: Liposomes developed herein are well suited for the application in the delivery of             L-cysteine. Particularly, they can encapsulate nearly all the L-cysteine and can retain it for 6 hours. Also, L-cysteine stabilized liposomes, preventing their aggregation. L-cysteine encapsulated in the DSPC formulation induced deeper changes in cell metabolism, causing a decrease in metabolic activity; this was probably due to a higher entry, thus a better liposome-mediated transport. Considering that the smaller the particle, the better the circulation, we believe that the stabilization of the vesicle by L-cysteine may allow these transporters to have higher circulation times. Based on the above, we conclude that the DSPC formulation is the best suited for further application in          L-cysteine delivery.

Liposomes L-cysteine FTIR cell metabolism biophysics

References (33)

  1. 1 Liposome: classification, preparation, and applications [DOI]
  2. 2 Liposomes as nanomedical devices [DOI]
  3. 3 Liposomes in drug delivery: Progress and limitations [DOI]
  4. 4 A review on phospholipids and their main applications in drug delivery systems [DOI]
  5. 5 Proteins, polysaccharides, and their complexes used as stabilizers for emulsions: Alternatives to synthetic surfactants in the pharmaceutical field? [DOI]
  6. 6 Nanoparticle-induced surface reconstruction of phospholipid membranes [DOI]
  7. 7 Liposomal drug formulations in cancer therapy: 15 years along the road [DOI]
  8. 8 Formation, characterization, and fate of inhaled drug nanoparticles [DOI]
  9. 9 Oxidative stress and ageing: is ageing a cysteine deficiency syndrome? [DOI]
  10. 10 Glutathione and infection [DOI]
  11. 11 Stability of Liposomal Formulations in Physiological Conditions for Oral Drug Delivery [DOI]
  12. 12 Long-term stability, biocompatibility and oral delivery potential of risperidone-loaded solid lipid nanoparticles [DOI]
  13. 13 Perturbations of membrane structure by optical probes: I. Location and structural sensitivity of merocyanine 540 bound to phospholipid membranes [DOI]
  14. 14 Stealth liposomes: an improved sustained release system for 1-beta-D-arabinofuranosylcytosine.
  15. 15 The structure and organization of phospholipid bilayers as revealed by infrared spectroscopy [DOI]
  16. 16 The Influence of Ionic Strength and Lipid Bilayer Charge on the Stability of Liposomes [DOI]
  17. 17 A calorimetric and spectroscopic comparison of the effects of ergosterol and cholesterol on the thermotropic phase behavior and organization of dipalmitoylphosphatidylcholine bilayer membranes [DOI]
  18. 18 Influence of HEK293 metabolism on the production of viral vectors and vaccine [DOI]
  19. 19 Redispersible liposomal-N-acetylcysteine powder for pulmonary administration: Development, in vitro characterization and antioxidant activity [DOI]
  20. 20 Phase Transitions of Binary Lipid Mixtures: A Combined Study by Adiabatic Scanning Calorimetry and Quartz Crystal Microbalance with Dissipation Monitoring [DOI]
  21. 21 Optimization and In Vivo Toxicity Evaluation of G4.5 Pamam Dendrimer-Risperidone Complexes [DOI]
  22. 22 The chain conformational order of ergosterol- or cholesterol-containing DPPC bilayers as modulated by Amphotericin B: a FTIR study [DOI]
  23. 23 Effect of Risperidone and Fluoxetine on the Movement and Neurochemical Changes of Zebrafish [DOI]
  24. 24 Effect of Cholesterol on the Phase Behavior of Solid-Supported Lipid Vesicle Layers [DOI]
  25. 25 Liposomal protection of adriamycin-induced cardiotoxicity in mice.
  26. 26 Hydration of the Dienic Lipid Dioctadecadienoylphosphatidylcholine in the Lamellar Phase–An Infrared Linear Dichroism and X-Ray Study on Headgroup Orientation, Water Ordering, and Bilayer Dimensions [DOI]
  27. 27 Liposome/DNA systems: correlation between association, hydrophobicity and cell viability [DOI]
  28. 28 Highly stable vesicles composed of a new chain-terminus acetylenic photopolymeric phospholipid [DOI]
  29. 29 Lipid-Polymer Membranes as Carriers for L-Tryptophan: Molecular and Metabolic Properties [DOI]
  30. 30 A Calorimetric and Spectroscopic Comparison of the Effects on Ergosterol and Cholesterol on the Thermotropic Phase Behavior and Organization of Dipalmitoylphosphatidylcholine Bilayer Membranes [DOI]
  31. 31 Liposome/DNA Systems: Correlation Between Hydrophobicity and DNA Conformational Changes [DOI]
  32. 32 Targeted Drug Delivery: A Two-Compartment Growth Inhibition Assay Demonstrates That Fluorodeoxyuridine and Fluorodeoxyuridine Monophosphate Are Liposome-Independent Drugs [DOI]
  33. 33 International Journal of Nanomedicine [DOI]

Cited by 14

Dual targeted magnetic photosensitive liposomes for photothermal/photodynamic tumor therapy

T.S. Anilkumar, Yu-Jen Lu, Huai-An Chen · Journal of Magnetism and Magnetic Materials · 2019

Encapsulation of an endostatin peptide in liposomes: Stability, release, and cytotoxicity study

Nastaran Rezaei, Faramarz Mehrnejad, Zahra Vaezi · Colloids and Surfaces B: Biointerfaces · 2020

Tripeptide-Loaded Liposomes as Multifunctional Components in Topical Formulations

Michał Dymek, Maria José García-Celma, Elvira Escribano-Ferrer · International Journal of Molecular Sciences · 2025

Garcinol-loaded Novel Cationic Nanoliposomes: In Vitro and In Vivo Study Against B16F10 Melanoma Tumor Model

Brahamacharry Paul, Raghuvir H Gaonkar, Ria Mukhopadhyay · Nanomedicine · 2019

Screening and enhancement of the antimicrobial activity of some plant oils using liposomes as nanoscale carrier

Maha G. Haggag, Medhat W. Shafaa, Hossam S. Kareem · Bulletin of the National Research Centre · 2021

Showing 7 of 14 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

44

Page views

0

PDF downloads

6

Outbound clicks

14

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.