DPPH and ABTS Radical Scavenging Assay and Antibacterial Efficacy of Methanol Flower Extract of Peltophorum pterocarpum (DC.) Backer ex K. Heyne

Abstract

Aim: The present study is to investigate the anti-radical activity and antibacterial potential of the methanol flower extract of Peltophorum pterocarpum. Methods: Anti-radical activity was done through the DPPH and ABTS radical scavenging assay. The antibacterial activity of methanol flower extract was tested against Bacillus subtilis and Escherichia coli bacteria by agar well diffusion method. Results: The methanol flower extract of P. pterocarpum demonstrated mild DPPH anti-radical activity and for ABTS radical scavenging assay presented remarkable antioxidant activity with corresponding IC50 values of 103.5μg/ml and 48.60μg/ml respectively. Quercetin was used as a standard. The crude flower extract showed a significant zone of inhibition (1.5 mm) against E. coli (a Gram-negative bacterium) at 30 μg/well, compared to a smaller zone of inhibition (1.2 mm) against B. subtilis (a Gram-positive bacterium). The positive control, streptomycin, produced zones of inhibition of 2.2 mm and 2.5 mm. respectively. Conclusion: The present findings confirms the existence of mild and remarkable antioxidant activity and substantial antibacterial potential in the flower extract of P. pterocarpum and this data could pave the way for further research study.