DNA Barcoding of Selected Freshwater Fishes in New Calabar River, Rivers State, Nigeria
Anukwu John Uchenna, Nwamba Helen Ogochukwu, Chiaha Ijeoma Emiliana
Asian Journal of Biotechnology and Bioresource Technology · pp. 28–38 · Published 20 Apr 2023
10.9734/ajb2t/2023/v9i1176Abstract
The major environmental crisis is the loss of biodiversity and the decline is predominant in the fish population. Although taxonomic history began 250 years ago, there are still undiscovered members of species and new species are waiting to be uncovered. The failure of the traditional taxonomic method to address this issue has resulted to the adoption of a molecular approach-DNA barcoding. It was proposed that DNA barcoding using the mitochondrion cytochrome oxidase subunit I (COI) gene has the capability to serve as a barcode for fish. The aim of this study was to use DNA barcoding in the identification of fish species in the New Calabar River, Rivers State. BLAST result showed the correlation between the sequence queried and the biological sequences with the NCBI database. The names of the samples, percentage ID, predicted organisms, and GenBank Accession numbers were clearly identified. A total of 18 sequences (all > 600bp) belonging to 8 species, 7 genera, 7 families, and 5 orders were validated and submitted to the NCBI database. Each nucleotide peak was represented by a single colour with various percentage occurrences. Two (22%) out of the 9 original samples analyzed corresponded with the predicted organisms from the BLAST result.) There were a total of 712 positions in the final dataset. Evolutionary analyses were conducted in MEGA11. Pairwise sequence alignment showed different consensus positions and a total of 30 mutations. There was one insertion from Polynemus dubius and 29 substitutions (transition-15 and transversion-14) mutations. No deletion and nonsense codons were detected in all the amplified sequences. This work will facilitate more research in other keys areas such as the identification of mislabeled fish products, illegal trading of endangered species, and effective tracking of fish biodiversity.
References (23)
- 1 Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [DOI]
- 2 DNA sequencing with chain-terminating inhibitors [DOI]
- 3 The neighbor-joining method: a new method for reconstructing phylogenetic trees. [DOI]
- 4 MEGA11: Molecular Evolutionary Genetics Analysis Version 11 [DOI]
- 5 DNA barcodes distinguish species of tropical Lepidoptera [DOI]
- 6 The Magnitude of Global Marine Species Diversity [DOI]
- 7 Identifying Canadian Freshwater Fishes through DNA Barcodes [DOI]
- 8 DNA barcoding Indian marine fishes [DOI]
- 9 Mislabelling of a depleted reef fish [DOI]
- 10 Barcoding Nemo: DNA-Based Identifications for the Ornamental Fish Trade [DOI]
- 11 Pigments, Patterns, and Fish Behavior [DOI]
- 12 Mechanical properties of a bio-inspired robotic knifefish with an undulatory propulsor [DOI]
- 13 DNA barcoding is a useful tool for the identification of marine fishes from Japan [DOI]
- 14 A reliable DNA barcode reference library for the identification of the North European shelf fish fauna [DOI]
- 15 Direct PCR amplification and sequencing of specimens’ DNA from preservative ethanol [DOI]
- 16 Cutting force of fibrous materials [DOI]
- 17 DNA barcoding for identification of fish species from freshwater in Enugu and Anambra States of Nigeria [DOI]
- 18 FishAPP: A mobile App to detect fish falsification through image processing and machine learning techniques [DOI]
- 19 Fish and seafood traceability based on AFLP markers: Elaboration of a species database [DOI]
- 20 A role for barcoding in the study of African fish diversity and conservation
- 21 Fully-automated identification of fish species based on otolith contour: using short-time Fourier transform and discriminant analysis (STFT-DA) [DOI]
- 22 Background matching ability and the maintenance of a colour polymorphism in the red devil cichlid [DOI]
- 23 Fully-automated identification of fish species based on otolith contour: using short-time Fourier transform and discriminant analysis (STFT-DA) [DOI]
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