Development of Low Erucic Acid Content Rapeseed Mutant Variety BINA Sarisha12

Abstract

Any plant breeding program must have genetic variability, and mutagenesis is a proven method of creating new variability. Gamma irradiation was used to create novel genetic variability in rapeseed at different doses. Recently released new rapeseed (Brassica napus) mutant variety, BINA sarisha12 developed from popular variety Binasarisha-9 by gamma irradiation. Though Binasarisha-9 is a high yielding variety but duration is around 90 days and erucic acid content is 39.14%. For developing early maturing and low erucic acid content new variety, initially the seeds of Binasarisha-9 were exposed to different doses of gamma rays (550, 650, 750, 800 and 900 Gy) and M1 was grown in 2013-14. Irradiated populations were evaluated in M2, M3 and M4 generations during 2014-15, 2015-16 and 2016-17, respectively. Selected mutants carried out of various types of yield trial in 2017-18, 2018-19 and 2019-20 in experimental farms and in the farmers’ fields also. Performance of selected three mutants, derived from 650 and 850 Gy of gamma rays (one from 650 and two from 850 Gy), were found to be superior to other mutant lines and mother variety Binasarisha-9. The mutant RM-005 grown in various places around the country following researcher and farmer’s management practices during 2020-21 and 2021-22. Comparative assessment done in respect of seed yield, maturity period, plant height, number of primary branches plant-1, number of siliqua plant-1, number of seeds siliqua-1 etc. Reactions to major disease (Alternaria blight), insect-pest (aphid) infestation and analyzing fatty acid profile also studied. Based on the superior performance of RM-005, like as, tolerant to Alternaria blight, showed the lower incidence of aphid infestation and low erucic acid content than the check variety, National Seed Board of Bangladesh registered this mutant as an improved rapeseed variety named BINA sarisha12.