Determination of Bioactive Compounds and Antimicrobial Capabilities of Purified Allium sativum Extract to Multidrug Resistant Enteric Bacteria
Journal of Advances in Microbiology · pp. 49–61 · Published 22 Mar 2021
10.9734/jamb/2021/v21i230327Abstract
There is much concern on spread of pathogenic enteric bacteria and there resistance to commercially available antibiotics. There is a need for development of a new reliable drug for the treatment of diseases caused by these bacteria. This study determines the antimicrobial capabilities of purified Allim sativum extract to multidrug resistant enteric bacteria. In-vitro antibacterial properties of the extract was carried out using agar well diffusion technique against antibiotics resistant enteric bacteria. The results revealed that Seven fractions of partially purified water extract of Allium sativum (fraction 5-11) had significant zone of inhibition to the test enteric bacteria. The Fourier Transform Infrared Spectrophotometer (FTIR) spectra revealed the presence of 24 functional groups in functional groups in water extract of A. sativum. Also, Gas Chromatography-Mass Spectrophotometry revealed the presence of different compounds in water extract of Allium sativum.
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