Detection of Antibiotic Resistance Genes among Pseudomonas aeruginosa Strains Isolated from Burn Patients in Iran
Ali Hashemi, Fatemeh Fallah, Soroor Erfanimanesh, Alireza Salimi Chirani, Masoud Dadashi
Microbiology Research Journal International · pp. 1–6 · Published 13 Jan 2016
10.9734/BMRJ/2016/23268Abstract
Aim: In this study, we evaluated the presence of antibiotic resistance genes among P. aeruginosa strains. Methodology: From January to September 2012, 100 isolates of P. aeruginosa were collected from burn patients. Antimicrobial susceptibility testing was performed by disk diffusion method. Screening for Metallo-β-lactamases (MBLs) productions were performed by Combination Disk Diffusion Test (CDDT). The frequency of antibiotic resistance encoding genes such as MBLs (IMP, VIM, NDM), ESBLs (CTX-M-15), Amp-C enzyme (CMY), Ambler class A carbapenemases (KPC), Ambler class D β-lactamase (OXA-48), 16S rRNA methylases (armA, rmtB, rmtC, rmtD), Quinolone Resistance Gene (aac(6′)-Ib) and class 1 integron were performed by PCR and Sequencing techniques. Results: 48(62.33%) of isolates were metallo-beta-lactamase producers. All MBL-producing P. aeruginosawere resistant to antibiotics; while 49% of isolates were resistant to Gentamicin. The aac(6)-Ib, CTX-M-15, int I, CMY, rmtB , rmtD and IMP-1 genes were detected in 57 (74.02%), 48 (62.3%), 48 (62.3%), 7 (9.09%), 11 (14.28%), 9 (11.68%) and 6 (7.7%) isolates respectively, whereas none of them were positive for other genes. The mortality rate due to metallo-β-lactamases-producing P. aeruginosa infection was 5(10.4%). Conclusions: The prevalence of antibiotic resistance genes producing P. aeruginosa detected in this study is of great concern.
References (15)
- 1 Multiplex PCR for detection of acquired carbapenemase genes [DOI]
- 2 Metallo-β-lactamases in Gram-negative bacteria: introducing the era of pan-resistance? [DOI]
- 3 Contribution of OqxAB efflux pumps to quinolone resistance in extended-spectrum- -lactamase-producing Klebsiella pneumoniae [DOI]
- 4 Antibiotic resistance and frequency of class 1 integrons among Pseudomonas aeruginosa, isolated from burn patients in Guilan, Iran.
- 5 Coproduction of Novel 16S rRNA Methylase RmtD and Metallo-β-Lactamase SPM-1 in a Panresistant Pseudomonas aeruginosa Isolate from Brazil [DOI]
- 6 Decellularized human amniotic membrane: more is needed for an efficient dressing for protection of burns against antibiotic-resistant bacteria isolated from burn patients [DOI]
- 7 Dissemination of a Class I Integron Carrying VIM-2 Carbapenemase in Pseudomonas aeruginosa Clinical Isolates from a Hospital Intensive Care Unit in Annaba, Algeria [DOI]
- 8 Detection of Ambler class A, B and D ß-lactamases among Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates from burn patients.
- 9 Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia [DOI]
- 10 Class 1 integron and Imipenem Resistance in Clinical Isolates of Pseudomonas aeruginosa: Prevalence and Antibiotic Susceptibility.
- 11 The First Report of CMY, aac (6′)-Ib and 16S rRNA Methylase Genes Among Pseudomonas aeruginosa Isolates From Iran [DOI]
- 12 Identification and Characterization of Metallo-β-Lactamases Producing Pseudomonas aeruginosa Clinical Isolates in University Hospital from Zanjan Province, Iran [DOI]
- 13 Carbapenem-Resistant Bacteria and Laboratory Detection Methods [DOI]
- 14 Antimicrobial Activity of Doripenem and Other Carbapenems Against Gram-Negative Pathogens from Korea [DOI]
- 15 Archives of Pediatric Infectious Diseases [DOI]
Cited by 12
L. Ahmadian, Zahra Norouzi Bazgir, M. Ahanjan · BioMed Research International · 2021
S. D. Costa-Júnior, Adriana Maria Costa Marques da Silva, Jussyêgles Niedja da Paz Pereira · Brazilian Journal of Microbiology · 2021
Niloofar Meamar, دJamshid Razmyar, S. M. Peighambari · 2021
M. Dadashi, Somayyeh Yaslianifard, Bahareh Hajikhani · Journal of Global Antimicrobial Resistance · 2019
A. Abaza, Soraya A. el Shazly, Heba S. A. Selim · Polish Journal of Microbiology · 2017
A. Abaza, Soraya A. el Shazly, Heba S. A. Selim · 2017
Mojtaba Kashfi, A. Hashemi, G. Eslami · 2016
Leila Shamkhali, M. Shahriari · 2021
Showing 8 of 12 known citations — external sources report more than can currently be individually listed.
Related research
- Antimicrobial Activity of Pyocyanin for Inhibition of Pseudomonas aeruginosa Urinary Tract Pathogens — shares topic coverage
- Dew Formation and Anaerobiosis in Anaerobic Jars — shares topic coverage
- Carbapenemase-Encoding Genes in Pseudomonas aeruginosa: Multiplex PCR Detection, Microarray Based Detection and Gene Sequencing — shares topic coverage
- Pseudomonas aeruginosa Infection in Humans and Antibiotic Resistance — shares topic coverage
- Examination of the Production of Biofilm and Acyl Homoserine Lactone (AHL) in Pseudomonas aeruginosa Isolates Exposed to Sub-inhibitory Antibiotics Concentrations — shares topic coverage
Article metrics
Real usage data collected on this platform.
10
Page views
0
PDF downloads
1
Outbound clicks
12
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.