Influence of Hormonal Treatments on STEM Cuttings through Multiplication in Therapeutically Worthwhile Plant Tinospora cordifolia

Abstract

India is a leader in the usage of the ancient ayurveda system and is blessed with a vast richness of medicinal herbs. Over 68 Ayurveda formulations contain the medicine Guduchi as one of its main constituents, and need for this medication has climbed from 3000 to 6000 MT with a 9.1% yearly increase. Unfortunately, uncontrolled harvesting is causing the natural populations to decline. There is plenty of room to expand the commercial production of these plants given the enormous increase in demand. For the purpose of creating a technique for rapid multiplication, propagation by stem cuttings was examined in the current experiment. For this species, the impact of various GA3 concentrations on stem cuttings during 30 DAP and 45 DAP was investigated using the randomized block design (RBD) with four replications. The 200 ppm GA3-treated stem cuttings of T. cordifolia (T1) showed  considerable rooting (85.75%). The highest shoot lengths at 45 days after planting were measured for treatments T1 (average 106.15 cm). Maximum root numbers were measured for treatments T1 (6.25 cm) and treatment T1 had slightly longer roots (17.42 cm), but there were no statistical differences in mean values. Throughout the course of the experiment, T. cordifolia cuttings' average lowest and maximum growth rates ranged from 4.33 cm/day to 7.1 cm/day. The results of this study showed that the optimal propagation approach for T. cordifolia for commercial multiplication is influence of hormonal treatments on stem cuttings.