Cytotoxicity Activity of Green Tea and Mint Formulation

Abstract

Background: Cytotoxicity refers to a substance or process which results in cell damage or cell death. The prefix ‘cyto’ refers to a cell and ‘toxic’ to poison. The  term is often used to describe chemotherapy drugs that kill cancer cells, but it may also be used to describe toxins, such as venom. Within our own immune system , we have cells that are considered cytotoxic, such as the T cell that kills bacteria, viruses, and cancer cells. Materials and Methods:  100ml of distilled water, 1 gram of green tea leaf and 1 gram of powdered mint is added and the mixture is heated for 15 to 20 minutes and then filtered with the help of filtered paper. The mixture was again heated and concentrated from 70ml to 20ml.  2 grams of iodine free salt was weighed and dissolved in 200ml of distilled water. 6 well ELISA plates were taken and 10-12ml of saline water was filled. To that 10 nauplii were added to each well( 5μl, 10μl, 20μl, 40μl, 80μl). Then the nanoparticles were added according to the concentration level. The plates were incubated for 24 hours. After 24 hours, the elisa plates were observed and noted for the number of live nauplii’s present. Results and Discussion: The cytotoxicity activity of green tea with mint formulation shows that all the introduced shrimps were alive in the control whereas it is shows that the lethality of 5μl is 60%, 10μl is 70%, 20μl is 70%, 40μl is 70% and 80μl is 100%. Conclusion: Based on the result of the study it is finally concluded that cytotoxic activity of green tea and mint formulation has better effect.