Characterization of Bacteria that Promote Growth from Spent Mushroom Compost and their Effectiveness on the Yield and Quality of White Button Mushrooms

Abstract

Micro-organisms in the substrate and mushroom casing are essential to the start and growth of primordial. For mushroom production, the discarded mushroom compost may be a useful source of bacteria that promote development. "Characterization of bacteria that promote growth from spent mushroom compost and their effectiveness on the yield and quality of white button mushrooms" is the title of the current study that was conducted. Spent mushroom compost (SMC) samples were gathered from various locations in Solan, including Chambaghat (DMR), Nauni (UHF), and local farmers (Dharja). In order to isolate the bacteria, nutrient agar medium was utilized. From discarded mushroom compost, 25 bacterial isolates from SMC were chosen and refined. Twelve of the twenty-five bacterial isolates (seven from Nauni, four from the local farmer site, and fourteen from Chambaghat) produced P-solubilizer, fifteen nitrogen fixers, nine HCN, thirteen siderophores, and ten IAA. Out of 25 bacterial isolates, only two (GPB1 and GPB2) were chosen based on different PGPR characteristics. Bacterial isolate NS2 produced the highest amounts of siderophores (96.37%) on solid CAS medium and (286.21μg/ml) in liquid assay, IAA (79.60 μg/ml), HCN, and P-solubilization both qualitatively (93.3%) and quantitatively (214.76 μg/ml). These two bacterial isolates (GPB1, GPB2) were tested for growth of white button mushroom in field experiment. Mushrooms innoculated with Treatment 7(GPB1+ GPB2 on casing and substrate) showed significant increase in yield (75 %) and biological efficiency in all three flushes over Uninoculated control. The treatment T7 (GPB1+ GPB2) registered 51.3 per cent increase in antioxidant enzyme assay content,67.83 per cent increase in protein content, 74.5 percent in carbohydrate content, 87.8 per cent increase in ash content over uninoculated control (T1). This treatment also increased NPK content of compost over uninoculated control. Hence these bacterial isolates have enormous potential to be used as biofertilizers for enhanced growth and quality of white button mushroom.