In-vitro Propagation of Aloe vera (L.) Burm. f
Sharmistha Gupta, Pankaj K. Sahu, Devki L. Sen, Priyanka Pandey
Biotechnology Journal International · pp. 806–816 · Published 12 Jul 2014
10.9734/BBJ/2014/9747Abstract
Department of Botany, Dr. C.V. Raman University, Bilaspur, (C.G.) India Aim: In vitro culture is used for commercial production and is achieved in aseptic condition using different concentration and combination of Plant Growth Regulators (PGR). Material and Methods: In present work studied the effects of different plant growth regulator singly or in combination in tissue culture of Aloe vera (L.) Burm. f. belongs to the family Liliaceae or Asphodelaceae, used in ayurveda as well as pharmaceutical industry. Murashige and Skoog (MS) media with different combinations and concentration of growth promoters i.e. Auxin (Indole-3-butyric acid (IBA), α-Naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinin (Benzyl Amino Purine (BAP) were used in this study. Surface sterilization was standardised with HgCl2 with various concentration and time. Observation and Result: The development of callus type was observed in the MS media supplemented with BAP for best regeneration, IBA for root formation and NAA, was found the best media for root formation (0.5mg/l) were seen to grow onwards from the tenth day of culture and 90% of root formation took place within a span of 3-4 weeks for maximum callus induction. The maximum number of root & shoot produced is 4.8±0.53 with average length of 3.5±0.35cm. Discussion: The present work deals with in vitro plant growth of Aloe vera through tissue culture for propagation and ex situ conservation. Regenerated plants after acclimatization were transferred to soil and they showed 85% survival. The culture response was maximum in apical buds (100%) and minimum time required is 9.67 days in the same media. The average length of shoots per culture was 4.0±0.16cm. In present study among the three types of auxins, NAA was found to be the best for root induction.
Cited by 12
Kshanika Hirimburegama, Rochana Piyumal · Biotechnological Production of Bioactive Phytochemicals of Medicinal Value · 2024
Giuseppe Cristiano, Bernardo Murillo-Amador, Barbara De Lucia · Frontiers in Plant Science · 2016
Department of Environmental Horticulture, Sahmyook University, Seoul, 01795, Korea, Raisa Aone M. Cabahug, Sang Yong Nam · Flower Research Journal · 2018
Awad Y. Shala, Hayam M. Elmenofy, Eman Abd El-Hakim Eisa · In Vitro Propagation and Secondary Metabolite Production from Medicinal Plants: Current Trends (Part 2) · 2024
Mebrahtom Welehaweria, Desta Berhe Sbhatu · BMC Research Notes · 2023
· Anti-Diabetes Mellitus Plants · 2016
Manokari M., Priyadharshini S., Mahipal S. Shekhawat · Industrial Crops and Products · 2021
Atsbeha Hailu, Desta Berhe Sbhatu, Haftom Baraki Abraha · The Scientific World Journal · 2020
Isha Fatima, Muhammad Akram, Hamid Mukhtar · Essentials of Medicinal and Aromatic Crops · 2023
Mulu Niguse, Desta Berhe Sbhatu, Haftom Baraki Abraha · The Scientific World Journal · 2020
Related research
- In vitro Impact Assessment of Aqueous Extract of Sida cordifolia Linn. Upon Rat Spermatozoa Parameters — shares topic coverage
- In vitro Probiotic Potential of Autochthonous Lactic Acid Bacteria and Microbiology of Kunu Made from Mixed Grains — shares topic coverage
- In vitro Investigation of Antimicrobial, Antitumor and DPPH Reduction Capacity of the Methanolic Extract of Scoparia dulcis — shares topic coverage
- Antifungal Efficacy of Zinc Oxide Nanoparticles against Anthracnose and Southern Blight Disease in Green Gram — shares topic coverage
- Evaluation of Fungicides against Sheath Rot Disease of Rice: An In vitro Study — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
12
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.