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Research Article Open access CC BY 3.0

Composition, Antioxidant, Antibacterial Activities and Mode of Action of Clove (Syzygium aromaticum L.) Buds Essential Oil

Hassan Barakat

Current Journal of Applied Science and Technology · pp. 1934–1951 · Published 12 Mar 2014

10.9734/BJAST/2014/8902

Abstract

The use of natural antimicrobial compounds, especially extracted from plants, as food preservatives is nowadays widely used, since plant matrices possesses antimicrobial and antioxidant natural products to protect themselves from microbial infection and deterioration as well as for human health benefit properties. The present work was undertaken to examine the antioxidant and antimicrobial activities of clove (Syzygium aromaticum L.) buds essential oil. Clove essential oil (CEO) exhibited high amount of total phenolic compounds (TPC) with high radical scavenging activity toward DPPH, ABTS and linoleic acid radicals as well as iron chelating activity. The TPC of CEO was 845 mg GAE g-1 demonstrated 509 µmol of TE g-1DPPH radicals scavenging activity and 713 µmol of TE g-1 ABTS radicals scavenging activity. The antioxidant capacity of CEO exhibited 94% reduction when evaluated by β-carotene bleaching assay. The Reducing power activity related to iron chelating was 314 µmol of AAE g-1. The composition of CEO exhibit high eugenol content (80.19%) over 16 identified components by GC-MS analysis. Furthermore, CEO exhibited antibacterial activity in vitro at low concentrations against tested food borne pathogens applying disc diffusion and microdilutions assays. The minimum inhibitory concentration values (MICs) for tested bacteria which were sensitive to CEO were in the range of 1400–3600µg ml-1. The phenolic components of CEO are most active and appear to act principally as membrane permeabilizers which established using staining-DNA fluorescence dye assay. Quantification of membrane permeabilization exudes a positive relationship between the cell membrane damaging and concentrations. Therefore, the CEO can be reliably used in commercial applications as antioxidant, antibacterial and flavoring agent in individual or in combination with common preservatives for controlling the undesirable organoleptic and microbial deteriorations in foodstuffs.

Syzygium aromaticum L. essential oil total phenolic compounds antioxidant activity antibacterial activity mode of action

References (60)

  1. 1 Antioxidant activity applying an improved ABTS radical cation decolorization assay [DOI]
  2. 2 Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents [DOI]
  3. 3 Principles and Procedures of Statistics: A Biometrical Approach
  4. 4 Antioxidant Determinations by the Use of a Stable Free Radical [DOI]
  5. 5 Antimicrobial activity of essential oils and other plant extracts [DOI]
  6. 6 Mechanisms of membrane toxicity of hydrocarbons [DOI]
  7. 7 A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol [DOI]
  8. 8 Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment [DOI]
  9. 9 The Relative Antioxidant Activities of Plant-Derived Polyphenolic Flavonoids [DOI]
  10. 10 Antibacterial and Antifungal Properties of Essential Oils [DOI]
  11. 11 Characterization of the Action of Selected Essential Oil Components on Gram-Negative Bacteria [DOI]
  12. 12 The Phenolic Hydroxyl Group of Carvacrol Is Essential for Action against the Food-Borne Pathogen Bacillus cereus [DOI]
  13. 13 Interactions of cyclic hydrocarbons with biological membranes. [DOI]
  14. 14 Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts [DOI]
  15. 15 Use of different methods for testing antioxidative activity of oregano essential oil [DOI]
  16. 16 Antioxidant activities of carotenes and xanthophylls [DOI]
  17. 17 Mechanism of Action of Melaleuca alternifolia (Tea Tree) Oil on Staphylococcus aureus Determined by Time-Kill, Lysis, Leakage, and Salt Tolerance Assays and Electron Microscopy [DOI]
  18. 18 The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata ( Syzigium aromaticum L. Myrtaceae): a short review [DOI]
  19. 19 Factors that interact with the antibacterial action of thyme essential oil and its active constituents [DOI]
  20. 20 Antibacterial activity of selected plant essential oils against Escherichia coli O157:H7 [DOI]
  21. 21 Mechanisms of Action of Carvacrol on the Food-Borne Pathogen Bacillus cereus [DOI]
  22. 22 Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri [DOI]
  23. 23 A simplified method for the evaluation of antioxidants [DOI]
  24. 24 Solid- and Vapor-Phase Antimicrobial Activities of Six Essential Oils: Susceptibility of Selected Foodborne Bacterial and Fungal Strains [DOI]
  25. 25 Antibacterial activity of extracts from some edible plants commonly consumed in Asia [DOI]
  26. 26 Eugenol—From the Remote Maluku Islands to the International Market Place: A Review of a Remarkable and Versatile Molecule [DOI]
  27. 27 Fluorometric assessment of Gram-negative bacterial permeabilization [DOI]
  28. 28 Antimicrobial activity of clove and rosemary essential oils alone and in combination [DOI]
  29. 29 Evaluation of Combined Antibacterial Effects of Eugenol, Cinnamaldehyde, Thymol, and Carvacrol against E. coli with an Improved Method [DOI]
  30. 30 Permeabilization of Fungal Membranes by Plant Defensins Inhibits Fungal Growth [DOI]
  31. 31 Alkylperoxyl Radical-Scavenging Activity of Various Flavonoids and Other Phenolic Compounds: Implications for the Anti-Tumor-Promoter Effect of Vegetables [DOI]
  32. 32 Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases [DOI]
  33. 33 Antibacterial activity of 11 essential oils against Bacillus cereus in tyndallized carrot broth [DOI]
  34. 34 Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia Ten. [DOI]
  35. 35 Antimicrobial activities of Eugenol and Cinnamaldehyde against the human gastric pathogen Helicobacter pylori [DOI]
  36. 36 Synergistic interaction of eugenol with antibiotics against Gram negative bacteria [DOI]
  37. 37 Influence of carvacrol on growth and toxin production by Bacillus cereus [DOI]
  38. 38 Inhibitory effect of beta-pinene, alpha-pinene and eugenol on the growth of potential infectious endocarditis causing Gram-positive bacteria [DOI]
  39. 39 Current Status of Metals as Therapeutic Targets in Alzheimer's Disease [DOI]
  40. 40 Evolution of Phenolic Compounds and Antioxidant Activity during Malting [DOI]
  41. 41 Effect of Syzygium aromaticum extract on immediate hypersensitivity in rats [DOI]
  42. 42 Effects of Turkish spice extracts at various concentrations on the growth of Escherichia coli O157:H7 [DOI]
  43. 43 Eugenol Reduces the Expression of Virulence-Related Exoproteins in Staphylococcus aureus [DOI]
  44. 44 Antimicrobial effect of spices and herbs on Vibrio parahaemolyticus [DOI]
  45. 45 Eugenol a valuable compound for in vitro experimental research and worthwhile for further in vivo investigation [DOI]
  46. 46 The in vitro antioxidative properties of the essential oils and methanol extracts of Satureja spicigera (K. Koch.) Boiss. and Satureja cuneifolia ten [DOI]
  47. 47 In vitro antimicrobial activity of essential oil from endemic Origanum minutiflorum on ciprofloxacin-resistant Campylobacter spp. [DOI]
  48. 48 Chemical composition and antimicrobial activity of the essential oil of Syzygium aromaticum flower bud (Clove) against fish systemic bacteria isolated from aquaculture sites [DOI]
  49. 49 New insights into the target site and mode of action of the antifungal protein of Aspergillus giganteus [DOI]
  50. 50 Antimicrobial effects of garlic, clove and red hot chilli onListeria monocytogenesin broth model systems and soft cheese [DOI]
  51. 51 Essential oils: their antibacterial properties and potential applications in foods—a review [DOI]
  52. 52 Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts [DOI]
  53. 53 THE USE OF THE STABLE FREE RADICAL DIPHENYLPICRYLHYDRAZYL (DPPH) FOR ESTIMATING ANTIOXIDANT ACTIVITY
  54. 54 A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents [DOI]
  55. 55 Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey [DOI]
  56. 56 The proposed mechanism of bactericidal action of eugenol, ∝-terpineol and g-terpinene against Listeria monocytogenes, Streptococcus pyogenes, Proteus vulgaris and Escherichia coli [DOI]
  57. 57 Effects of selected plant essential oils on the growth and development of mouse preimplantation embryos in vivo [DOI]
  58. 58 Biotechnologically relevant enzymes and proteins [DOI]
  59. 59 GC-MS Study On The Potentials Of Syzygium aromaticum
  60. 60 Antimicrobial Activity of Cinnamate-eugenol: Synergistic Potential, Evidence of Efflux Pumps and Amino Acid Effects [DOI]

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