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Research Article Open access CC BY 4.0

Comparative Study of the Effect of Two Extraction Solvents on Polyphenols Content and Antioxidant Activity of Alstonia Boonei Bark

Marielle Anastasie Zali, Martin Fonkoua, Charles Dakole Daboy, William Arnold Tazon, David Goda, Dupon Ambamba Akamba, Jules Vidal Nanhah, Judith Laure Ngondi

Asian Journal of Chemical Sciences · pp. 7–17 · Published 15 Jul 2023

10.9734/ajocs/2023/v13i4245

Abstract

Oxidative stress (OS) is the imbalance between the generation of reactive oxygen species (ROS) and the body's antioxidant defenses in favor of ROS. ROS are chemical species that can be neutralized by natural phytochemicals like phenolic compounds present in medicinal plants and whose extraction depends on the solvent system used. Thus, the present study aimed to evaluate the effect of two extraction solvents on the polyphenols content and antioxidant activity of Alstonia boonei barks. For this, the bark of A. boonei were harvested, processed, dried and ground, then the powder was macerated in two solvent systems (water and water-ethanol). Subsequently, the quantitative phytochemical analysis of the different extractions was done by evaluating total polyphenols content (TPC), total flavonoids content (TFC) and alkaloids; followed by an in vitro evaluation of the antioxidants activity through radicals scavenging (DPPH and NO.) and the reducing power of ferric iron to ferrous iron. The relationship between antioxidant potential (DPPH, NO and FRAP) and polyphenols (total polyphenols and total flavonoids) was investigated using simple linear regression. It appears that the aqueous extract allowed better extraction of phenolic compounds from A. boonei bark; and exhibited better antioxidant activity. In addition, a strong correlation (r and R2) was found between polyphenols (TPC and TFC) and antioxidant activity (DPPH, NO and FRAPP). Thus, we preferably recommend the use of water as a solvent for traditional preparations of A. Boonei bark extract for therapeutic purposes.

Solvents polyphenols antioxidant Alstonia Boonei bark

References (14)

  1. 1 Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids [DOI]
  2. 2 First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid [DOI]
  3. 3 Antioxidant and prooxidant properties of flavonoids [DOI]
  4. 4 Significance of HbA1c Test in Diagnosis and Prognosis of Diabetic Patients [DOI]
  5. 5 Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus [DOI]
  6. 6 Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts [DOI]
  7. 7 Antioxidant effectiveness of selected wines in comparison with (+)-catechin [DOI]
  8. 8 Environmentally Friendly Methods for Flavonoid Extraction from Plant Material: Impact of Their Operating Conditions on Yield and Antioxidant Properties [DOI]
  9. 9 Dietary antioxidants remodel DNA methylation patterns in chronic disease [DOI]
  10. 10 Comparison of the total phenolic content, total anthocyanin content and antioxidant activity of polyphenol-rich fruits grown in Chile [DOI]
  11. 11 Effect of extraction solvents on polyphenols and antioxidant activity of medicinal halophytes
  12. 12 Antiplasmodial activity of stem bark and leaves of Alstonia boonei (De Wild) [DOI]
  13. 13 Antioxidant and Glucose Lowering Effects of Hydroethanolic Extract of Baillonella toxisperma Pulp [DOI]
  14. 14 Aqueous Extract of Alstonia boonei Bark Reduces Chronic Hyperglycemia and Prevents its Complications through Increase of Hepatic Global Dna Methylation in Diabetic Wistar Rats [DOI]

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