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Research Article Open access CC BY 4.0

Evaluation of the Most Effective Plant Extracts, Bioagents and Chemical Fungicides In vitro against Powdery Mildew of Pea

A. V. Bhosale, V. M. Karade, C. T. Kumbhar, R. M. Khadtare, V. M. Rajenimbalkar, S. S. Shinde

Annual Research & Review in Biology · pp. 1–6 · Published 20 Feb 2024

10.9734/arrb/2024/v39i330633

Abstract

The present investigation was carried during 2022-23 at Plant Pathology Section, College of Agriculture, Pune-05 with the objectives of evaluation of the most effective plant extracts, bioagents and chemical fungicides in vitro. Effect of six plant extracts, bioagents and chemical fungicides were studied in vitro by hanging drop method where the study revealed that garlic (Allium sativum), followed by neem (Azadiracta indica) showed highest inhibition of spore germination for powdery mildew. After 48 hours of incubation, the spore germination rate ranged from 3.15 % to 34.45 %, where garlic showed the highest inhibition at 95.30 %, followed by neem at 91.03 %. The efficacy of bioagents was evaluated by preparing the bacterial and fungal culture filtrates where Trichoderma harzianum and Pseudomonas fluorescens were found to be the most effective with tune of 56.86% and 50.72% of efficacy respectively against Erysiphe pisi. Among all chemical fungicides, hexaconazole (0.05 %) resulted as the most effective chemical fungicide against powdery mildew of pea. These findings can be useful in developing safer and more eco-friendly methods to control powdery mildew and protect crops.

Pea powdery mildew conidia spore inhibition bioagents plant extracts fungicides

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