Characteristics and Antibiogram Studies of Bacteria Associated with Vegetables Stored In Raffia Baskets in Nigeria
D. N. Ogbonna, S. I. Douglas, M. E. Inana
Journal of Applied Life Sciences International · pp. 1–17 · Published 7 Nov 2019
10.9734/jalsi/2019/v22i230124Abstract
Raffia baskets are used in rural communities to preserve fresh vegetables for a given period of time. This method of storage is aimed at extending the shelf life of the vegetables until they are used. Thus, this study was aimed at determining the microbial succession during storage and susceptibility pattern of bacterial isolates from stored leafy vegetables to various antibiotics. Five (5) leafy vegetables namely, Bitter leaf (Vernomia anydalira), Water leaf (Talinum triangulare), Fluted pumpkin leaf, (Telfairia occidentalis), Okazi leaf (Gnetum africana) and Scent leaf (Ocimum gratissimum) were obtained from the Nigerian Stored Products Research Institute (NSPRI) farm, Port Harcourt. These vegetables were stored in a raffia basket for fourteen (14) days while another set of the vegetables were left in the open air as control. This set up was monitored for changes to occur. Standard microbiological techniques were employed for the various analyses of the vegetable samples. Both cultural and molecular characterizations of the isolates were done. The disc diffusion method was used in determining the susceptibility pattern of the bacterial isolates. The results of the predominant microorganisms identified were of the genus; Bacillus, Pseudomonas, Enterococcus, Enterobacter, Bordetella, Staphylococcus, Myroides, Escherichia, Serratia, Micrococcus and Acetobacter. Also, Bacillus species occurred in all the vegetables while Enterococcus faecalis, Acetobacter orientalis, Bordetella pertussis, Myroides xuanwuensis and Bacillus flexus were isolated on the third day of storage from the vegetables. The total heterotrophic bacterial counts ranged from 1.8 x106 cfu/g to 1.25 x107 cfu/g (bitter leaf), 9x105 cfu/g to 9.0 x106 cfu/g (Scent leaf), 7 x105 cfu/g to 1.88 x107 cfu/g (Okazi), 3.2x106 cfu/g to 1.05x107 cfu/g (Fluted pumpkin leaf) and 8.4 x106 cfu/g to 2.04 x107 cfu/g (water leaf). Antibiogram of bacterial isolates revealed that they were resistant to Augmentin and Ceftazidime according to Clinical Laboratory Standard Institute schemes. Combination of the antibiotics showed that most of the isolates were 100% susceptible to ciprofloxacin + erythromycin, and ciprofloxacin + ceftriaxone. The study revealed that for all the vegetables, those stored in the basket lasted longer and were fresher compared to those kept in the open space. This study revealed that the raffia baskets are suitable for storage and preservation of leafy vegetables, but microbial succession occurred as a result of increase in the period of storage leading to the proliferation of microorganisms. This could be from aerosols or by chance inoculation, through handling or materials used or other microorganisms which are inherent in the vegetables because of their contact with the soil or water used in the washing of the leaves.
References (10)
- 1 Assessment of bacteriological quality of ready to eat food (Meat pie) in Benin City metropolis, Nigeria [DOI]
- 2 The microbial quality of pre-packed mixed vegetable salad in some retail outlets in Lagos, Nigeria. [DOI]
- 3 Crònica legislativa de Catalunya. Segon semestre de 2025. “Un període marcat per les noves invectives judicials contra el marc normatiu lingüístic a l’escola i per iniciatives polítiques com el Pacte Local pel Català, la formació dels nouvinguts i els drets lingüístics en l’àmbit sociosanitari” [DOI]
- 4 Isolation and Identification of Fungi Associated with the Spoilage of Sweet Orange (Citrus Sinensis) Fruits In Sokoto State [DOI]
- 5 African fermented foods: from art to science [DOI]
- 6 Comparative Assessment of Phytochemicals, Proximate and Elemental Composition of Gnetum africanum (Okazi) Leaves [DOI]
- 7 Evaluation of Indoor Air for Bacteria Organisms and their Antimicrobial Susceptibility Profiles in a Government Health Institution [DOI]
- 8 Characterization and Multiple Antibiotic Resistance of Bacterial Isolates Associated with Fish Aquaculture in Ponds and Rivers in Port Harcourt, Nigeria [DOI]
- 9 Microbiological Quality of Hoplobatrachus occipitalis (Amphibia, Anura) Used as Meat [DOI]
- 10 Microbial flora and nutritional composition of adult Palm - Wine Beetle (Rhychophorus phoenicus)
Cited by 2
Soibibo Alabo Harry, Janet Olufunmilaya Williams, Salome I. Douglas · South Asian Journal of Research in Microbiology · 2023
S. Musa · International Journal of Advanced Research · 2021
Related research
- Antibiotic Susceptibility Pattern of Group A Beta-Haemolytic Streptococcal Throat Isolates of Primary School Children in Rivers State, Nigeria — shares topic coverage
- Prevalence, Aetiological Agents and Antimicrobial Resistance Patterns of Urinary Tract Infections among Pregnant Women Attending Mother and Child Hospital, Akure, South-West Nigeria — shares topic coverage
- Incidence of Enterobacteriaceae in Retail Meat Samples and Their Antibiotic Susceptibility — shares topic coverage
- Bacterial Aetiology and Antibiotic Susceptibility Profile of Post-Operative Sepsis among Surgical Patients in a Tertiary Hospital in Rural Eastern Uganda — shares topic coverage
- Traditional and Molecular Identification of Bacterial Contamination from Honey (Processed and Unprocessed) and Their Control by Phytotherapy — shares topic coverage
Article metrics
Real usage data collected on this platform.
51
Page views
0
PDF downloads
21
Outbound clicks
2
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.