Skip to content
Research Article Open access CC BY 4.0

Characterisation and In-vitro Fungicide Susceptibility of Corynespora cassiicola Associated with Leaf Spot Disease of Aglaonema Ruby Garuda

Sandra S. R., Gleena Mary C. F., Reshmy Vijayaraghavan, Mini Sankar, Sible George Varghese, Mufeeda K. T., Shambhu Kumar

Journal of Advances in Biology & Biotechnology · pp. 625–637 · Published 22 May 2024

10.9734/jabb/2024/v27i6922

Abstract

During 2020- 2022, a characteristic leaf spot symptom was recorded from the Aglaonema cultivar, Aglaonema Ruby Garuda Aglaonema Ruby Garuda from Ambunadu region, Ernakulam district of Kerala. The symptomatic plants formed dark brown, water-soaked, circular spots with concentric zonations and recorded 30 per cent disease severity. Pathogen associated with the symptom isolated using standard isolation methods and pathogenicity of the fungal isolates was established. Greyish brown mycelial growth was observed in culture plates with greyish black colour on the reverse side. Conidia of the fungus were sub hyaline to pale brown, solitary, straight to slightly curved, obclavate produced on brown septate mycelia with a dimension of 27.3-47.8 µm × 4.2-12.8 µm. The amplicon sequences of internal transcribed spacer (ITS) and translational elongation factor 1- alpha (TEF1-α) regions of the pathogen were analysed with the nucleotide sequences in nBLAST database and identified as Coryenespora cassiicola. Evolutionary relationship of the pathogen with related organisms were identified by constructing phylogenetic tree using ITS and TEF1-α sequences. In vitro efficacy of 11 chemical fungicides at different dosages and three biocontrol agents tested recorded, cent per cent inhibition with contact fungicides viz. mancozeb, propineb, Bordeaux mixture. Complete inhibition was observed with systemic fungicides, hexaconazole 5EC, tebuconazole 25.9EC, carbendazim 50WP at all test dosages. Among the combination fungicides, carbendazim 12%+ mancozeb 63% was superior with cent per cent mycelial inhibition. Among the biocontrol agents, the fungal antagonist, Trichoderma asperellum (KAU reference culture) recorded complete inhibition against Coryenespora cassiicola by dual culture assay.

Aglaonema Ruby Garuda Leaf spot disease Corynespora cassiicola In-vitro evaluation poisoned food technique dual culture technique

References (21)

  1. 1 MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms [DOI]
  2. 2 AMPLIFICATION AND DIRECT SEQUENCING OF FUNGAL RIBOSOMAL RNA GENES FOR PHYLOGENETICS [DOI]
  3. 3 A method for designing primer sets for speciation studies in filamentous ascomycetes [DOI]
  4. 4 Distortion of Fungal Hyphæ in the Presence of Certain Inhibitors [DOI]
  5. 5 A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay [DOI]
  6. 6 Diseases of Crop Plants in India
  7. 7 Soybean disease loss estimates for the United States from 1996 to 1998 [DOI]
  8. 8 Trichoderma asperellum T1 mediated antifungal activity and induced defense response against leaf spot fungi in lettuce (Lactuca sativa L.) [DOI]
  9. 9 Potential of PGPR in Agricultural Innovations [DOI]
  10. 10 Methods for Research on the Ecology of Soil-Borne Plant Pathogens [DOI]
  11. 11 Descriptions of Pathogenic Fungi and Bacteria [DOI]
  12. 12 Development of New Foliage Plant Cultivars
  13. 13 PGPR: a good step to control several of plant pathogens. [DOI]
  14. 14 PATHOGENICITY AND EPIDEMIOLOGY OF CORYNESPORA CASSIICOLA IN THE REPUBLIC OF THE SEYCHELLES [DOI]
  15. 15 A revision of the genus Aglaonema (Araceae) [DOI]
  16. 16 First Report of Corynespora cassiicola Causing Fruit Rot of Pomegranate in India, Its Morphological and Molecular Characterization [DOI]
  17. 17 Rapid Detection of Pectolytic Erwinia sp. in Aglaonema sp. [DOI]
  18. 18 Fungicides in Plant Disease Control
  19. 19 Anthracnose disease of Painted evergreen [Aglaonema crispum (Pitcher & Manda) Nicolson] caused by Colletotrichum gloeosporioides from West Bengal
  20. 20 A New Aglaonema Foliar Blight and Crown Rot
  21. 21 A study on biology of anthracnose from Aglaonema modestum Schott et Engl. and its control.

Cited by 2

First occurrence of Corynespora cassiicola infecting chia plant in Bangladesh and sensitivity of the pathogen to selected fungicides

Avi Kumar Badhon, D. R. Gupta, Swapan Kumar Paul · PLoS ONE · 2026

Showing 1 of 2 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

12

Page views

0

PDF downloads

2

Outbound clicks

2

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.