Micropropagation Via Embryogenesis Techniques for Reforestation of Valuable Teak (Tectona grandis Linn. f.)

Abstract

Introduction: Teak is a valuable woody timber trees in industry. Aim: Teak (Tectona grandis Linn. f.) is an important valuable timber value. Teak trees grow slowly with a harvesting cycle of 15-20 years and the seed germination rate is low. tissue culture is a method of rapid propagation of teak trees. The current manuscript gives an idea for propagating teak trees through micro propagation via embryogenesis techniques. The current manuscript gives an idea for propagating teak trees through micro propagation via embryogenesis techniques. Methods: By using plant cell technology. The basic medium MS (MS + Ascorbate (5 mg L-1) + vitamin B5 (5 mg L-1) + Ca-panthothenate (4 mg L-1) + glutamine (100 mg L-1) + CW (10%) favored for embryogenesis culture was used for whole experiments. Results: Young shoot tip, leaves, and roots were used to initiated cultures. Callus cells were initiated on MS + BA (1 mg L-1) + Ki (1 mg L-1) + NAA (0,5 mg L-1) after 30 days. Callus cells were differentiated to pro-embryogenic callus cells after subcultured on semi-solid basic medium MS + 2.4D (1 mg L-1) after 30 days. Pro-embryonic callus cells sourced from shoot tip were seperated on liquid basic medium MS + BA (0,5 mg L-1) + TDZ (0,2 mg L-1) + NAA (0.2 mg L-1) after 14 days. Pro-embryogenic callus cells were multiplied on liquid basic medium MS + BA (0,5 mg L-1) + TDZ (0,2 mg L-1) + TDZ (0.2 mg L-1) after 30 days. Pro-embryogenic callus cells were differentiated into embryogenic cells on layering basic medium MS + BA (0,5 mg L-1) + TDZ (0,2 mg L-1) + NAA (0.2 mg L-1) after 30 days. The conditioned basic medium MS + BA (0,5 mg L-1) + TDZ (0.2 mg L-1) + NAA (0,1 mg L-1) was required for stimulation process before transfering embryogenic cells to regeneration media. Shoots were regenerated on medium MS + BA (0,5 mg L-1) + TDZ (0.2 mg L-1) + NAA (0,1 mg L-1). A system for regeneration of teak somatic embryogenesis culture was established. Plantlets from embryos were used as materials to avoid degeneration for microprpagation and reforestation. Conclusion: A pilot scale for avoid of degeneration, regeneration and micro propagation system using somatic embryo technology has been established.