In vitro Antioxidant and Antibacterial activity of Bridelia atroviridis (Arasado)

Abstract

Aim: Bridelia atroviridis methanolic leaf extracts was assessed for antioxidant and antibacterial activity. Place and Duration of Study: The study was carried out in the Microbiology Laboratory, Department of Science Laboratory Technology, School of Pure and Applied Science, Lagos State Polytechnic, Ikorodu, Lagos- Nigeria for the period of three months between September and November 2015. Methodology: Antioxidant compounds lycophene, β-carotene, total phenolic and total flavonoid content were evaluated using the method described by Nagata and Yamashita, aluminum chloride colorimetric assay and Folin-Ciocalteau assay respectively. DPPH radical scavenging activity method was utilized in assessing the antioxidant capacity of the plant extract while the antimicrobial activity was evaluated by the agar diffusion technique. Results: The Bridelia atroviridis methanolic leaf extracts assessed in this study possessed significant amount of antioxidant compounds lycophene, β-carotene, total phenol and flavonoids. The extract exhibited antioxidant activity by scavenging DPPH radicals in a dose dependent pattern with IC50 of 51.24 µg/mL compared to vitamin C with IC50 of 41.67 µg/mL. Conclusion: Bridelia atroviridis methanolic leaf extracts is a potential source of drugs with antioxidant and antimicrobial activity.