Studies on Optimization of Culture Conditions and Medium Components for the Production of Mycelial Biomass of Auricularia delicata under Submerged Fermentation

Abstract

Aims: To optimize the culture conditions and medium components for the production of mycelial biomass of A. delicata under submerged fermentation. Place and Duration of Study: China–Zambia Agricultural demonstration center and Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, China between July 2019 and June 2020. Methodology: In this study, a single factor at a time method was employed in the optimization of submerged culture conditions and medium components for the production of mycelial biomass of A. delicata (strain YD 99). Each factor was screened independently while other factors were kept constant. Results: The findings of this study demonstrate that the optimal culture conditions obtained were as follows: carbon source (Glucose) 20 gl-1, pH 6.0, nitrogen source (Yeast extract) 2 gl-1, mineral elements (K2HPO4+MgSO4.7H20) 2gl-1, and incubation temperature 25°C. The application of these optimal culture conditions produced a maximum concentration of 7.34gl-1 mycelial biomass of A. delicata. Conclusion: Consequently, our results indicated that the optimization of culture conditions and medium components is of significant importance for the cultivation of A. delicata.