Phytochemical Evaluation and TLC Profile of Leaf and Bark in Nyctanthes arbor–tristis L.

Abstract

This research work mainly deals with the collection of plants, the extraction of active compounds from the bark and leaves. The Introductory phytochemical screening of Nyctanthes arbor–tristis L. revealed that about 20 phytochemicals present out of the 21 phytochemicals were tested in the various solvent extract like Ethanol, Petroleum ether, Chloroform. In petroleum ether the 8 phytochemicals were found. The chloroform extract shows the presence of 11 phytochemicals and in ethanol 3 phytochemicals were found like carbohydrate, alkaloid and cardiac glycoside. All the solvent extract exhibits the occurrence of Carbohydrates, Alkaloids, Saponins while Emodin was totally absent in all kinds of extract. The compounds like alkaloids, glycosides, saponins, steroids, flavonoids have expectorant (cough out) action which is very useful in the management of upper respiratory tract inflammation. During performance of TLC, the extracted solvents were used in reducing sequence of polarity. In each one of them extract many of the solvents with their polarity the active secondary metabolites were observed. Coloured spots have been seen directly when performed mobile phase versus the stationary phase in TLC chamber. Apart from this the colourless secondary metabolites were visualized by spraying the TLC plates with specific reagent like Ammonia, Iodine regent and Dragandroff’s reagent. The outcome of the performance of TLC profile showed that there are no similarities of Retention Factor (Rf) values among the plant extracts. On the basis of that finding all types of extract have different concentration of different kinds of phytochemicals as well as their rate of dissolution in different extracts.