Evaluation of Antiparkinson Activity of Vitamin C, Tizanidine and Pregabalin against Haloperidol Induced Parkinsonism in Rats

Abstract

The present study deals with the antiparkinson effect of Levodopa/carbidopa, Vitamin C, Tizanidine, Pregabalin on haloperidol (1 mg/kg i.p) induced Parkinsonism in rats. Neural degeneration was induced in animals (Group II, III, IV, V, VI, VII, VIII) by i.p. administration of haloperidol at 1mg/kg. Positive control (Group-III) treated by levodopa/carbidopa  (125 mg/kg p.o.), Test 1 (Group -IV) treated by vitamin C (120mg/kg p.o.), Test 2 (Group-V) treated by Tizanidine  (0.3 mg/kg i.p.), Test 3 (Group -VI) treated by Pregabalin  (30 mg/kg p.o.), Test 4 (Group-VII) treated by Vitamin C(120 mg/kg p.o.) and  Tizanidine (0.3 mg/kg i.p.) and Test 5 (Group-VIII) treated by Vitamin C(120 mg/kg p.o.) and  Pregabalin (30mg/kg p.o) daily were given before 30 min of haloperidol challenged for 14 days. Behavioural parameters were recorded on day 14 for high bar test, actophotometer test, rotarod test. MDA level and Dopamine level were also recorded. Test 4 (Group- VII) showed more significant reduction in cataleptic score. In actophotometer, Test 4 (Group-VII) showed significant increase in locomotor activity. In rotarod test, Test 2 (Group-V) and test 4 (Group-VII) showed more significant increase motor coordination activity. Levodopa/Carbidopa showed more significant increase in dopamine level and significantly abolished cataleptic score. Vitamin C showed more significant decrease in MDA level. It can be concluded that the tizanidine and pregabalin may possess antiparkinsonian activity. Vitamin C and tizanidine may possess synergestic effect. These results showed that tizanidine and pregabalin may be used to improve catalepsy in Parkinson disease.